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DnaJ诱导的miRNA-146a对巨细胞中IL-8的表达产生负面调节

Jaehoo Lee¹, Jinwon Huh², Yeji Lee²

¹Department of Biotechnology and Bioinformatics, Korea University, Sejong, 30019, Republic of Korea; Interdisciplinary Graduate Program for Artificial Intelligence Smart Convergence Technology, Korea University, Sejong, 30019, Republic of Korea.

Microbial pathogenesis

|September 16, 2023

概括

Pseudomonas aeruginosa DnaJ 蛋白质是一种热冲击蛋白,激活NF-κB信号传递,并在巨细胞中调节微RNA-146a (miR-146a). 这种miR-146a随后在P. aeruginosa感染期间抑制过度IL-8表达.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
微生物学微生物学

背景情况:

热冲击蛋白 (HSP) 是关键的损伤相关的分子模式,涉及天生的免疫力.
伪omonas aeruginosa (P. aeruginosa) 是一个重要的细菌病原体.
介乐-8 (IL-8) 是炎症反应中的一个关键细胞因子.

研究的目的:

调查P. aeruginosa DnaJ (HSP40同源) 在调节巨细胞IL-8表达中的作用.
阐明参与DnaJ介导免疫反应的信号通路和分子机制.
确定DnaJ对微RNA-146a (miR-146a) 表达的影响及其随后对P. aeruginosa诱导的炎症的影响.

主要方法:

用细菌热冲击蛋白 (DnaJ,DnaK,GroEL,HtpG) 处理的巨细胞培养物.
使用分子试验对IL-8和microRNA-146a表达的分析.
对NF-κB信号通路激活的研究.
使用miR-146a抑制剂和P. aeruginosa感染进行实验操纵.

主要成果:

通过NF-κB激活,P. aeruginosa DnaJ显著诱导IL-8表达比其他HSP更多.
DnaJ治疗显著增加了巨细胞中的miR-146a表达.
用DnaJ进行预治疗降低了P. aeruginosa诱导的IL-8水平,这种效果取决于miR-146a.

结论:

P. aeruginosa DnaJ通过NF-κB通路作为IL-8的强有力的诱导剂.
DnaJ对miR-146a进行上调,miR-146a作为IL-8的负调节剂起作用.
这项研究揭示了一种新的机制,即DnaJ诱导的miR-146a在P. aeruginosa感染期间调节过度的IL-8产生.

关键词:

DnaJJ 在线观看它们的IL-8和IL-8是不同的.伪omonas aeruginosa 这种类型的病毒.这就是miRNA-146a.

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