聚烯微塑料对细胞和生物能效的影响,取决于细胞类型,分化状态和暴露后的时间
Miao Peng1, Maaike Vercauteren1, Charlotte Grootaert2
1Laboratory of Environmental Toxicology and Aquatic Ecology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium; Blue Growth Research Lab, Ghent University, Wetenschapspark 1, 8400, Oostende, Belgium.
Environmental pollution (Barking, Essex : 1987)
|September 16, 2023
概括
微塑料 (MPs) 可以对人体细胞造成长期伤害,甚至在一次暴露后也会引起慢性压力和线粒体功能障碍. 这些影响持续长时间,突出了无处不在的MP污染带来的潜在健康风险.
科学领域:
- 环境健康科学 环境健康科学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 微塑料 (MP) 在食品和个人护理产品中普遍存在,引发了人们的健康问题.
- 关于MP效应的持续时间和它们对细胞分化的依赖性的数据有限.
研究的目的:
- 研究MPs对代表主要暴露路径 (肺,结肠,肝脏) 的人类细胞系的细胞和生物能效.
- 评估不同暴露时间和细胞分化状态对MP毒性的影响.
主要方法:
- 暴露不分化和分化肺 (A549,BEAS-2B),结肠 (Caco-2) 和肝脏 (HepG2) 细胞在不同度 (10^2-10^5颗粒/毫升) 的聚乙烯 (PS) MPs (2-μm) 中48小时和12天.
- 评估细胞吸收,细胞和线粒体功能,以及反应性氧物种 (ROS) 生产.
主要成果:
- 不分化的Caco-2细胞在短时间暴露 (48h) 中显示出高PS吸收,没有显著的细胞/线粒体影响.
- 生物效应在很大程度上独立于分化状态,尽管分化的细胞内化了较少的PS.
- PS诱导了长期的细胞和线粒体功能障碍,增加了氧化应激,并在暴露后长达14天内,在细胞 (包括细胞核) 中显示出高的保留率.
结论:
- 单次暴露于微塑料 (PS) 可以导致慢性压力和对人体细胞的有害影响,并产生长期影响.
- 微塑料可以在细胞内,包括细胞核,长时间存在,这表明潜在的累积损伤.
- 这项研究为评估微塑料在低度和各种暴露场景中的健康风险提供了基础.
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