血清长非编码RNA H19/微RNA-675-5p轴作为炎症性肠病的可能诊断生物标志物
Olfat G Shaker1, Aya Safa1, Ahmed Khairy2
1Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Giza, Egypt.
Molecular biology reports
|September 16, 2023
概括
长非编码RNA H19和microRNA-675-5p显示了炎症性肠病 (IBD) 的诊断潜力. 这些生物标志物,特别是H19和miRNA-675-5p,在性结肠炎和克罗恩病患者中升高,有助于诊断.
科学领域:
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
- 生物标志物发现发现
背景情况:
- 长非编码RNAs (lncRNAs) 和microRNAs (miRNAs) 都与炎症性肠病 (IBD) 的发生有关.
- 研究IncRNA H19和miRNA-675-5p为IBD的新型诊断标记提供了潜在的潜力.
研究的目的:
- 评估血清lncRNA H19和miRNA-675-5p在IBD患者的诊断效用.
- 评估这些生物标志物与性结肠炎 (UC) 和克罗恩病 (CD) 中的疾病活性之间的相关性.
主要方法:
- 使用定量实时PCR (qPCR) 来测量lncRNA H19和miRNA-675-5p的血清水平.
- 该研究包括97名参与者:35名UC患者,32名CD患者和30名IBD-free对照.
- 采用了接收器操作特征 (ROC) 分析和后勤回归.
主要成果:
- 与对照组相比,HC19和miRNA-675-5p的血清水平在UC和CD组显著升高 (P<0.0001).
- 在UC患者中,生物标志物水平与疾病严重程度相关,在中度病例中高于缓解.
- 对H19和miRNA-675-5p的ROC分析显示出高灵敏度和特异性,用于区分IBD亚型与对照.
结论:
- lncRNA H19和miRNA-675-5p作为IBD的有价值的诊断生物标志物.
- 这些生物标志物在性结肠炎患者的诊断和活动评估方面特别有前途.
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