乌斯蒂拉戈·梅迪斯 (Ustilago maydis) 类似于PR-1的蛋白质已经进化出了两个不同的域,用于双重毒性活动
Yu-Han Lin1, Meng-Yun Xu1, Chuan-Chih Hsu1
1Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 115201, Taiwan.
Nature communications
|September 16, 2023
概括
类的PR-1类蛋白质感知类物质并触发生长. 乌斯蒂拉戈梅迪斯使用宿主酶来切割其类似PR-1的蛋白质,释放抑制植物免疫力和促进感染的.
科学领域:
- 植物病原体相互作用
- 病毒性的分子机制
- 蛋白质结构与功能之间的关系.
背景情况:
- 病原体通过共同进化的过程进化了效应器功能,以克服宿主防御.
- 植物致病相关蛋白质 (PR) 和PR-1类蛋白质 (PR-1L) 分别在植物免疫和真菌毒性中发挥作用.
- 菌PR-1L蛋白质抵消植物防御的确切机制在很大程度上是未知的.
研究的目的:
- 研究真菌PR-1类蛋白在宿主-病原体相互作用中的功能.
- 为了阐明Ustilago maydisUmPR-1La如何逃避植物防御机制.
- 了解玉米B型甲素3 (CatB3) 在真菌病原发生中的作用.
主要方法:
- 对UmPR-1La和酵母ScPRY1.1进行比较分析.
- 对细胞表面定位和感应能力的研究.
- 生物化学试验研究由CatB3.3进行的蛋白质裂变.
- 评估CAPE类的活性和对植物免疫力的影响.
主要成果:
- PR-1La和ScPRY1通过富含Ser/Thr的区域定位到细胞表面,并感知基.
- UmPR-1La在检测类物质和诱导真菌中状生长方面表现出专门的活动.
- 梅迪斯利用玉米CatB3将UmPR-1La切割到一个CNYD动机上,释放出类似CAPE的.
- 这些可以破坏植物的CAPE原始免疫力,增强真菌的毒性.
- CatB3选择性地分裂UmPR-1La,避免具有类似动机的植物PR-1蛋白质.
结论:
- 菌PR-1L蛋白质在感知环境线索和调节宿主免疫力方面具有双重作用.
- U. maydis已经发展出一种复杂的机制,涉及宿主蛋白酶来抑制植物防御.
- UmPR-1La的专业功能对真菌病原性和宿主殖民有显著的贡献.
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