从乳制品和水样中分离出的Vibrio物种的分子表征
Mona A El-Zamkan1, Ahmed Shaban Ahmed2, Hanan H Abdelhafeez3
1Department of Food Hygiene and Control (Milk Hygiene), Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt. m_zam@vet.svu.edu.eg.
Scientific reports
|September 16, 2023
概括
在生牛奶,乳制品和水中检测到包括Vibrio cholerae和Vibrio parahaemolyticus在内的Vibrio物种. 许多分离物体表现出青素耐药性,并携带病毒性基因,构成食品安全风险.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 公共卫生 公共卫生
背景情况:
- 菌种类是重要的食源性病原体,导致胃肠道疾病.
- 食物和水源受到Vibrio的污染引起了公众健康的关注.
研究的目的:
- 在生牛奶,乳制品和水中检测出Vibrio cholerae和Vibrio parahaemolyticus.
- 为了研究病毒毒性因素,抗生素耐药性和生物膜的形成,在孤立的Vibrio菌株.
主要方法:
- 传统和分子方法用于细菌识别.
- 进行了抗生素耐药性分析,MAR指数计算和病毒性基因查.
主要成果:
- 在5%的样本中发现了菌物种;Vibrio cholerae和Vibrio parahaemolyticus分别在1.25%和1.5%中发现.
- 所有的Vibrio分离物都表现出青素耐药性 (MAR指数为0.16-0.5).
- 病毒性基因 (ctxAB,hlyA,tcpA) 在Vibrio cholerae中普遍存在,而tdh+/trh+基因在Vibrio parahaemolyticus中普遍存在.
结论:
- 在食品和水样本中存在抗生素耐药性和毒性Vibrio物种,突出了潜在的食物传播感染风险.
- 监测和控制措施对于减轻乳制品和水源中的振动菌污染至关重要.
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