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Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
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实践研究注射条件在渐变化模式下对治疗性蛋白质分析的影响,当使用非常短的列时

Raquel Pérez-Robles1, Szabolcs Fekete2, Natalia Navas3

  • 1Instituto de Investigación Biosanitaria de Granada (ibs.GRANADA), Granada, Spain; Department of Analytical Chemistry, Science Faculty, University of Granada, Granada, Spain; Fundación para la Investigación Biosanitaria de Andalucía Oriental-Alejandro Otero, Granada, Spain.

Journal of chromatography. A
|September 17, 2023
PubMed
概括

对于超短的RPLC和IEX列来说,当分离诸如治疗性蛋白质等大分子时,可以进行大体积注射. 然而,HILIC柱需要最小的注入量,以防止峰值扭曲和突破效应.

关键词:
装载能力 装载能力注射量量注射量是如何使用的离子交换色谱学 离子交换色谱学一个单克隆抗体.蛋白质是一种蛋白质.超短的列 超短的列

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