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通过超分辨率光显微镜对PEGylated mesoporous silica颗粒的蛋白质冠状体进行空间映射和定量评估

Nan Sun¹, Yi Jia¹, Shiwei Bai²

¹Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Journal of colloid and interface science

|September 17, 2023

概括

聚乙烯甘醇 (PEG) 修改有效地减少了纳米颗粒上的蛋白质冠状形成. 增加PEG链长度显著抑制蛋白质吸附,这对于生物医学应用中的纳米粒子安全性和有效性至关重要.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
表面化学表面化学

背景情况:

纳米粒子 (NP) 与生物流体相互作用,形成蛋白质冠状,改变它们的生物行为.
聚乙烯甘醇 (PEG) 修饰是一种减少蛋白质冠状形成的常见策略.
对PEG对冠状蛋白的抑制作用的定量分析是有限的.

研究的目的:

为了直接观察和量化PEGylated中孔性二氧化颗粒上的蛋白质冠状.
研究PEG分子量和化时间对蛋白质透的影响.
了解细胞命运和蛋白质冠状病毒的局部化.

主要方法:

直接随机光学重建显微镜 (dSTORM) 用于可视化蛋白质冠状病毒.
蛋白质透深度的量化.
使用细胞标记物 (溶解体) 的同局部化研究.

主要成果:

蛋白质透深度显示,随着化时间的延长,蛋白质透深度略有增加.
增加PEG链长度显著降低了蛋白质透深度.
证明了蛋白质冠状体与 lysosomes 的共同定位,表明细胞吸收途径.

结论:

dSTORM提供了一种强大的工具,用于在PEGylated NP上表征蛋白冠状.
PEG链长度是控制蛋白质冠状形成的关键因素.
了解蛋白质冠状动力学对于设计安全有效的基于的纳米材料用于生物医学至关重要.

关键词:

半孔性二氧化颗粒的部分.通过PEGylation进行化.透的深度透的深度蛋白质冠状病毒的冠状病毒超高分辨率成像成像技术视觉化的可视化

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