对花粉素细胞的microRNA转录组分析预测,诺奇和胰岛素通路会影响的卵泡发育
Manman Shen1, Mingzhu Wang2, Dehui Li2
1College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China; Jiangsu Key Laboratory of Animal Genetic Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China; Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, 225125, China.
Theriogenology
|September 17, 2023
概括
这项研究确定了调节卵巢毛囊发育的关键微RNA (miRNA). 这些发现为了解小RNA在家禽繁殖中的作用和改善产卵性能提供了基础.
科学领域:
- 动物科学动物科学
- 分子生物学分子生物学
- 生殖生物学 生殖生物学
背景情况:
- 微RNAs (miRNAs) 是繁殖的关键调节者.
- 颗粒状细胞 (GC) 的发育影响毛囊层次结构和产卵性能.
- 识别参与卵泡发育的miRNA对于家禽科学至关重要.
研究的目的:
- 确定调节性miRNA及其控制毛囊发育的生物机制.
- 在不同毛囊阶段的粒状细胞中分析差异表达的miRNA (DEMs).
- 探索信号通路在miRNA介导的毛囊发育中的参与.
主要方法:
- 来自小黄色毛囊 (SYFG),F6G和F1G的GCs的小RNA测序.
- 在毛囊阶段对miRNAs的差异表达分析.
- 目标基因预测和途径丰富分析 (MAPK,TGF-β,Wnt,Notch,胰岛素信号).
- 对PSEN2表达的RT-qPCR验证.
主要成果:
- 在SYFG.vs.F6G,SYFG.vs.F1G和F6G.vs.F1G的比较中分别确定了99,196和110个DEM.
- 在所有阶段发现了22种常见的DEM,包括miR-223,miR-103a,miR-449c-3p和miR-203a.
- 预测MAPK,TGF-β,Wnt,Notch和胰岛素信号通路的参与.
- 证实了PSEN2表达的显著变化,这是Notch和胰岛素信号的调节者.
结论:
- 这项研究提供了对调节毛囊发育的miRNAs的全面数据集.
- 已识别的miRNA和途径为调节毛囊成熟的分子机制提供了洞察力.
- 这些发现为未来关于家禽繁殖和产卵特征中的miRNA功能研究奠定了基础.
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