多焦点肺状细胞癌的基因组分期独立于综合形态评估
Sanja Dacic1, Xuanye Cao2, Neus Bota-Rabassedas2
1Department of Pathology University of Pittsburgh, Pittsburgh, Pennsylvania.
概括
使用形态学的多焦点肺状细胞癌 (LSCCs) 的分期是不可靠的. 整体外基因组测序 (WES) 是一种更准确的方法,用于在LSCC中将单独的原发性肺癌 (SPLC) 与肺内转移 (IPM) 区分开来.
科学领域:
- 在瘤学瘤学.
- 基因组学就是基因组学.
- 病理学 病理学 病理学
背景情况:
- 精确分期多焦点肺状细胞癌 (LSCCs) 是至关重要的,但由于有限的形态和分子数据具有挑战性.
- 区分单独的原发性肺癌 (SPLC) 和肺内转移 (IPM) 对于有效的治疗策略至关重要.
研究的目的:
- 评估整个外体序列 (WES) 作为分阶段多焦点LSCC的黄金标准.
- 将基因组资料与全面的形态评估进行比较,以将LSCC分类为SPLC或IPM.
主要方法:
- 在20个多焦点LSCC瘤对上进行了整体外体序列测定 (WES).
- 基因组数据分析了克隆和亚克隆突变,并与16名胸部病理学家的组织学评估进行了比较.
- 使用HTG EdgeSeq精密免疫瘤学小组进行了免疫基因分析.
主要成果:
- 据WES统计,有11个病例被归类为SPLC,7个是IPM.
- 观察到显著的基因组和免疫原异质性,免疫基因表达与突变概况相关.
- 被归类为IPM的瘤显示出高百分比的共享突变 (33.5%80.7%).
- 形态学评估显示,只有58.3%的人同意WES,病理学家之间的观察者间一致性是公平的 (kappa = 0.232).
结论:
- 对分期多焦点LSCC的形态评估是不可靠的.
- 建议进行全面的基因组分析,如WES,以准确地确定多焦点LSCC的阶段.
相关概念视频
02:21Cancers Originate from Somatic Mutations in a Single Cell
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
02:21Cancers Originate from Somatic Mutations in a Single Cell
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...