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使用分子印记的化改性纤维素微粒对±-flurbiprofen进行基质分辨

Huda S AlSalem¹, M Monier², May Abdullah Abomuti³

¹Department of Chemistry, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.

International journal of biological macromolecules

|September 17, 2023

概括

研究人员开发了新的化-纤维素微粒,用于选择性捕获S-flurbiprofen (S-FP). 这些分子打印的聚合物有效地分离了±-flurbiprofen (FP),为奇拉药物分离提供了一种有前途的方法.

科学领域:

生物聚合物的化学化学
螺旋式分离螺旋式分离
药品分析药品分析

背景情况:

弗鲁比 (FP) 是一种非类固醇抗炎药物 (NSAID),存在两种反原体:具有抗炎活性的S-FP和具有微不足道作用的R-FP.
对于治疗疗效和最小化副作用而言,FP的酶选择性分离至关重要.
现有的性分离方法可能缺乏工业应用的效率或可扩展性.

研究的目的:

开发分子印制的微粒,用于弗鲁尔比烯反体的优先吸收和奇拉分辨率.
为了合成和描述一种用于选择性S-FP结合的新型酸-纤维素 (CHD) 生物聚合物.
为了评估enantioselective吸附剂的容量,亲和力和性能在奇拉分辨率.

主要方法:

乙烯纤维素 (CECN) 的合成,然后转化为二烯纤维素 (CHD).
制造CHD微粒并使用S-FP打印使用甲交叉链接.
对S-FP印制微粒 (S-FPCHD) 的表征和最佳结合条件 (pH) 的确定.
对S-FP和R-FP的分离选择性吸附能力和亲和力的评估.
在分隔列中应用S-FPCHD,以获得 (±) -FP的奇拉分辨率.

主要成果:

开发的S-FPCHD吸附剂在5-7的最佳pH范围内显示出125mg/g的高吸附能力.
与S-FP相比,S-FPCHD对S-FP具有显著的亲和力,其吸收率是R-FP的12倍.
在分离柱中进行的状分辨率实验显示,加载溶液中的R-FP的异构过量 (ee) 为93%,溶液中的S-FP为95%.

结论:

新型的水素-纤维素分子印制微粒 (S-FPCHD) 已成功开发用于反选择性弗卢比烯分离.
这种S-FPCHD吸附剂对S-flurbiprofen具有出色的选择性和能力,其性能优于其R-enantiomer.
这种方法提供了一个有效的方法,用于 (±) -flurbiprofen的奇拉分辨率,在制药分析和药物开发中具有潜在的应用.

关键词:

纤维素纤维素是一种纤维素.形的决议形的决议分子印记是一种分子印记.

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