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miR135a的使用可通过防止在脑缺血期间TRPM7的激活来改善脑缺血损伤

P Cepparulo¹, P Brancaccio¹, R Sirabella¹

¹Division of Pharmacology, Department of Neuroscience, School of Medicine, University of Naples Federico II, Naples, Italy.

CNS neuroscience & therapeutics

|September 18, 2023

概括

微RNA 135a (miR135a) 通过降低TRPM7.7的调节来保护大脑缺血. 这项研究表明,miR135a输液可以减少缺血损伤,并改善大鼠的神经功能.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
生物化学生物化学

背景情况:

不调节的阴离子流入有助于神经细胞在缺血期间死亡.
暂时的受体潜力 melastatin 7 (TRPM7) 通道介于缺血性大脑中的病理性 Ca2 + 过载.
基于microRNA (miRNA) 的疗法在神经退行性疾病方面表现有前途.

研究的目的:

评估miRNA135a在脑缺血中的作用.
研究miR135a对缺血性脑损伤和神经功能的影响.
为了确定miR135a是否通过调节TRPM7表达来发挥其作用.

主要方法:

通过RT-PCR,西斑和FISH测试在缺血性大鼠大脑皮层中评估miR135a和TRPM7表达.
在接受过渡性中脑动脉封闭 (tMCAo) 的老鼠中进行了脑内静脉 (icv) miR135a输液.
治疗后量化缺血体积和评估神经功能.

主要成果:

在大鼠大脑皮层缺血后72小时,miR135a的表达上调.
脑内静脉输注miR135a显著减少缺血损伤和改善神经结果.
miR135a输液导致了缺血性大脑中TRPM7蛋白水平的下调.

结论:

miR135a在脑缺血中起着保护作用.
抑制TRPM7,可能由miR135a介导,是缺血性中风的可行的治疗策略.
针对TRPM7激活的早期干预在脑缺血期间提供神经保护.

关键词:

TRPM7 TRPM7 是一个很好的方法.大脑缺血症的大脑缺血症离子恒温是离子恒温.miR1355 这是一个很好的选择.神经保护神经保护

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...