LINC00668通过miR-518c-3p/TRIP4轴促进非小肺癌的进展
Zhibin Lu1,2,1, Zhichao Xiao2,1, Qi Wang1,3,1
1The First Clinical Medical College of Nanjing Medical University, Nanjing, Jiangsu, China.
Cancer biomarkers : section A of Disease markers
|September 18, 2023
概括
长非编码RNA LINC00668通过向miR-518c-3p/TRIP4通路来促进非小细胞肺癌 (NSCLC) 的进展. 抑制LINC00668抑制NSCLC细胞增殖和转移,提供了一个潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 基因规则 基因规则
背景情况:
- 非小细胞肺癌 (NSCLC) 是癌症相关死亡的主要原因.
- 识别新的分子标对于推进NSCLC诊断和治疗策略至关重要.
研究的目的:
- 研究LINC00668在NSCLC细胞的生物功能中的作用.
- 阐明LINC00668在NSCLC进展中的潜在分子机制.
主要方法:
- 使用RT-qPCR,西斑,IHC和FISH进行基因表达分析.
- 细胞功能测试包括CCK8,殖民地形成,伤口愈合,Transwell和亡测试.
- 涉及RIP和双化酶试验的分子机制研究证实了LINC00668,miR-518c-3p和TRIP4之间的相互作用.
主要成果:
- 发现LINC00668在NSCLC组织和细胞系中显著上调.
- 沉默LINC00668抑制NSCLC细胞的增殖,迁移和入侵,同时促进细胞亡.
- LINC00668直接针对miR-518c-3p,这反过来又调节TRIP4的表达,形成了LINC00668/miR-518c-3p/TRIP4轴.
结论:
- LINC00668通过miR-518c-3p/TRIP4路径作为NSCLC进展中的瘤基因起作用.
- 这项研究揭示了LINC00668在NSCLC中的作用的新机制.
- 这些发现为针对性NSCLC治疗提供了潜在的见解.
更多相关视频
13:18Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma
Published on: March 3, 2023
1.3K
09:40Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
Published on: August 2, 2024
2.7K
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
02:07Tumor Progression
6.4K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.4K
01:20The Retinoblastoma Gene
4.1K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
01:22PI3K/mTOR/AKT Signaling Pathway
3.6K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.6K