黄金标准:一个完整的标准,便携式和互操作的MoClo工具,用于模型和非模型蛋白质细菌
Blas Blázquez1,2, David San León1,2, Jesús Torres-Bacete1
1Department of Systems Biology, Centro Nacional de Biotecnología, CSIC, Madrid, Spain.
Nucleic acids research
|September 18, 2023
概括
黄金标准 (GS) 是一种新的模块化克隆方法,用于合成生物学,可以在大多数细菌中实现标准化的遗传电路组装. 这种方法提高了生物零件的互操作性和可重复使用性.
科学领域:
- 合成生物学 合成生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 模块化克隆在合成生物学中至关重要,但缺乏标准化,导致不兼容的系统.
- 特定生物体的克隆方法阻碍了无的互操作性和广泛采用.
研究的目的:
- 引入黄金标准 (GS),一种IIS型组装方法,用于跨多种细菌物种进行标准化的模块化克隆.
- 为了实现大型遗传电路的组合组合,并促进生物部分和图书馆的可重复使用性.
主要方法:
- 基于IIS类限制酶和标准欧洲向量架构的金标准 (GS) 模块化克隆系统的开发.
- 在各种蛋白质细菌 (α-, β-, γ-, δ-) 中验证GS用于DNA组装,可移植性,互操作性和表型工程.
- 创建一个用于零件表征的计算管道和一个用于社区驱动开发的网络门户.
主要成果:
- 在不同的细菌中,GS成功实现了模块化克隆和多达20个转录单元的组合组装.
- 该方法证明了高DNA组装效率,可移植性和跨不同细菌宿主的互操作性.
- 通过计算表征管道验证GS部件的性能.
结论:
- 黄金标准 (GS) 为合成生物学中模块化克隆提供了一个开放的,标准化的框架,适用于大多数细菌.
- 它促进了复杂的遗传电路的创建,并促进了生物部件和组装结构的高效重复使用.
- 开发的资源,包括一个网页门户网站,旨在促进由社区驱动的合成生物学进步.
相关概念视频
01:26Bacterial Phylum Proteobacteria
40
Proteobacteria, one of the largest and most diverse bacterial phyla, encompasses a wide range of Gram-negative bacteria distinguished by their outer membrane composed of lipopolysaccharides. These microorganisms exhibit various metabolic capabilities, including phototrophy, chemolithotrophy, and heterotrophy, and thrive in diverse environments from soil to aquatic systems and host-associated niches. The phylum is divided into six classes: Alphaproteobacteria, Betaproteobacteria,...
40
01:28Prokaryotic Cells
35.1K
Prokaryotes are small unicellular organisms that include the domains — Archaea and Bacteria. Bacteria include many common microorganisms, such as Salmonella and E. coli, while the Archaea include extremophiles that live in harsh environments, such as volcanic springs.
Like eukaryotic cells, all prokaryotic cells are surrounded by a plasma membrane, have genetic material in the form of single, circular DNA, a cytoplasm that fills the interior of the cell, and ribosomes that synthesize...
Like eukaryotic cells, all prokaryotic cells are surrounded by a plasma membrane, have genetic material in the form of single, circular DNA, a cytoplasm that fills the interior of the cell, and ribosomes that synthesize...
35.1K
01:24Microbial Classification System
43
Classification is the process of organizing organisms into hierarchically inclusive groups based on their phenotypic similarities or evolutionary relationships. A species comprises one or more strains, and closely related species are grouped into genera. Genera are further classified into families, families into orders, orders into classes, and so forth, up to the domain level, which is the broadest taxonomic rank derived from a combination of phenotypic and genotypic data.The nomenclature of...
43
01:29Microbial Morphologies
43
Bacterial and archaeal cells exhibit remarkable diversity in shape and structure, critical in their adaptability and functionality. Among bacteria, the most commonly observed shapes include cocci and bacilli. Cocci are spherical and may exist singly or in groupings such as pairs (diplococci), chains (streptococci), clusters (staphylococci), or tetrads. Bacilli, in contrast, are rod-shaped and can also occur as single cells, in pairs, or chains, depending on their environmental and genetic...
43
01:29Modern Molecular Taxonomy
47
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
47
01:24Bacterial Phylum Tenericutes
40
The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...
40