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Emmanuel Albuquerque-Souza^1,2, Benjamin Shelling¹, Min Jiang¹

¹Department of Periodontics, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Molecular oral microbiology

|September 18, 2023

概括

Fusobacterium nucleatum是一种与牙周炎相关的细菌,可以诱导细胞衰老,这是一个衰老过程,损害了组织修复. 这一发现表明,一种新的机制有助于与年龄相关的牙周病进展.

科学领域:

微生物学微生物学
细胞生物学细胞生物学
老年学是一门学科.

背景情况:

牙周炎的患病率随着年龄的增长而上升,但相关细菌在衰老过程中的作用仍然不清楚.
Fusobacterium nucleatum与牙周炎和其他与年龄相关的疾病有关.
细胞衰老是衰老的一个关键标志,它降低了组织的修复能力.

研究的目的:

为了调查Fusobacterium nucleatum是否会在牙上皮细胞中触发细胞衰老.
探索F. nucleatum可能诱导衰老的机制.
评估F. nucleatum诱导的衰老对牙细胞修复能力的影响.

主要方法:

长期暴露于牙上皮细胞的F. nucleatum.
在基因和蛋白质水平上分析细胞循环停止标记物 (p16INK4a,p14ARF,pRb,p53,p21).
通过与衰老相关的β-galactosidase (SA-β-Gal) 活性评估 lysosomal 功能障碍.
通过测量 Lamin-B1 表达式来评估核外完整性.
使用伤口愈合试验对细胞修复的功能性评估.

主要成果:

长时间暴露于F. nucleatum会增加亲衰老标志物 (p16INK4a,p21,pRb) 和SA-β-Gal活性.
F.核减少了抗衰老标志物的表达 (p14ARF,p53) 和Lamin-B1.1.
细菌暴露后,在牙上皮细胞中观察到受损的伤口愈合能力,与衰老相关.
这些变化表明由F. nucleatum引起的类似衰老的表型.

结论:

菌核酸诱导了牙上皮细胞的亲衰老反应.
这种细菌介导的衰老可能会通过降低修复能力来损害牙周组织的平衡.
这些发现表明,一种潜在的机制将F. nucleatum与老化过程中对牙周炎的敏感性增加联系在一起.

关键词:

拉米尼-B1B1 的使用.衰老的衰老衰老的衰老循环林依赖的激酶抑制剂角质细胞 (keratinocytes) 是一种细胞.牙周炎是指牙周炎的一种疾病.衰老是一种老化.

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