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在小鼠模型中对抗aquaporin 5自身抗体产生的要求

Sabin Acharya¹, Ahreum Lee¹, Hyunjin Kim¹

¹Department of Immunology and Molecular Microbiology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.

Molecular oral microbiology

|September 18, 2023

概括

外来B细胞和T细胞表位因子对于通过驱动生殖中心反应来产生针对aquaporin-5 (AQP5) 的自身抗体至关重要. 这解释了为什么Prevotella melaninogenica (Pm) 通常不会在健康个体中触发这些自身抗体.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
这是一种自身免疫力.

背景情况:

像Prevotella melaninogenica (Pm) 这样的口腔细菌具有类似于人类AQP5.5的水素蛋白 (AQP).
人类AQP5是一种关键的水通道蛋白质,与Sjogren综合征有关.
了解针对AQP5的自身抗体的抗原触发因素对于自身免疫性疾病研究至关重要.

研究的目的:

调查诱导针对AQP5"E"表位体 (AQP5E) 的自身抗体的抗原要求.
阐明B细胞和T细胞表位在针对AQP5.5的自身抗体产生中的作用.
在健康个体中解释细菌AQP和人类AQP5之间的有限交叉反应.

主要方法:

用来自Pm AQP和人类AQP5.5的各种基免疫C57BL/6小鼠.
在排水淋巴结中分析生殖中心 (GC) B细胞和毛囊辅助T细胞.
流细胞计,以评估T细胞扩张和抗体产生.

主要成果:

一种由Pm衍生的 (PmE-L) 具有外来B细胞和T细胞表位素,有效诱导了抗AQP5E自身抗体和GC反应.
缺乏T细胞表位体或含有自身T细胞表位体的不能诱导显著的自身抗体产生.
即使有外来T细胞表位,自我免疫也没有增强自身抗体诱导.
斯特雷普塔维丁复合促进了B细胞反应,但限制了GCB细胞扩张和自身抗体诱导.

结论:

抗AQP5E自身抗体的产生需要外来B细胞和T细胞表位,以刺激AQP5特异性B细胞中强大的GC反应.
这种机制解释了在免疫反应中对像Pm.这样的口腔细菌的免疫反应期间,不经常产生交叉反应性抗AQP5自身抗体.

关键词:

水族 5 5 水族 5 自动抗体是一种自身抗体.这是一个细菌性ortolog.发芽中心的发芽中心.鼠标模型鼠标模型鼠标模型自我容忍的自我宽容.

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相关概念视频

Aquaporins

Aquaporins

Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.