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通过RNase E激活RhlB的Allosteric激活会诱导基质RNA中的部分双重开放

Heidi Zetzsche¹, Laura Raschke¹, Boris Fürtig¹

¹Center for Biomolecular Magnetic Resonance (BMRZ), Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-Universität, Frankfurt, Germany.

Frontiers in molecular biosciences

|September 18, 2023

概括

大肠杆菌的DEAD-Box螺旋酶RhlB解开RNA以进行降解. 结合RNase E可以增强RhlB的作用.

科学领域:

分子生物学分子生物学
生物化学生物化学
结构生物学结构生物学

背景情况:

大肠杆菌的DEAD-Box螺旋酶RhlB解结构化mRNA以使RNA通过降解体降解.
RNase E 相互作用在全质上刺激 RhlB 的 ATPase 和 RNA 解活动.
在RNase E结合时RhlB的结构变化及其对反应速率的影响尚不清楚.

研究的目的:

研究RhlB的RNase E激活的结构基础.
描述RNase E对RhlB的RNA结合亲和力和解活动的影响.
探索RNase E对不同RNA基质的RhlB激活机制.

主要方法:

核磁共振 (NMR) 谱学是指核磁共振的光谱学.
以RNA为中心的生物化学分析.
对具有不同拓的RNA基质进行分析.

主要成果:

结合RNase E增强了RhlB对特定RNA基质的亲和力,增加了ATP的周转率.
在RNase E的存在下,RhlB会诱导形状变化和RNA复合体的部分开放,5'-重挂,即使没有ATP.
这种激活机制在DEAD-Box螺旋酶中是独一无二的,它挑战了解的ATP结合先决条件.

结论:

结合RNase E激活RhlB通过一种新的机制,包括增强RNA亲和力和构造变化.
这些发现揭示了DEAD-Box酶激活的独特模式,与标准的依赖ATP的途径不同.
这项研究为RNA代谢和降解在大肠杆菌中的调节提供了新的见解.

关键词:

核磁共振光谱法 (NMR) 是一种光谱法.RNA的动态与RNA的动态在RNA折叠过程中.这种RNA基酶是RNA基酶.实时NMR是指实时的NMR.

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