Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Water: A Bronsted-Lowry Acid and Base02:30

Water: A Bronsted-Lowry Acid and Base

50.7K
The reaction between a Brønsted-Lowry acid and water is called acid ionization. For example, when hydrogen fluoride dissolves in water and ionizes, protons are transferred from hydrogen fluoride molecules to water molecules, yielding hydronium ions and fluoride ions:
50.7K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.2K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Calcium channel CNGC20 and calmodulin 7 regulate melatonin-induced calcium signaling and aphid resistance in watermelon.

Plant physiology·2026
Same author

Induction of Synthetic Apomixis in Two Sorghum Hybrids Enables Seed Yield and Genotype Preservation Over Multiple Generations.

Plant biotechnology journal·2025
Same author

ClPS1 gene-mediated manipulation of 2n pollen formation enables the creation of triploid seedless watermelon.

Molecular horticulture·2025
Same author

The HD-ZIP I transcription factor ClLL1 regulates lobed leaf development through the auxin pathway in watermelon.

The Plant journal : for cell and molecular biology·2025
Same author

Nitric oxide cross-links calcium signals to enhance cold tolerance via inhibiting calmodulin expression in watermelon.

Plant physiology·2025
Same author

The Ca2+ channels CNGC2 and CNGC20 mediate methyl jasmonate-induced calcium signaling and cold tolerance.

Plant physiology·2025
Same journal

Exploring the jasmonic acid signaling pathway: the role of <i>CsAOS</i> in modulating trichome density in cucumber.

Horticulture research·2026
Same journal

<i>StSAUR31</i> functions as a negative regulator of enzymatic browning in potato (<i>Solanum tuberosum</i> L.).

Horticulture research·2026
Same journal

Genomic divergence, adaptation, and the genetic basis of quality traits in ancient walnut landraces and wild relatives.

Horticulture research·2026
Same journal

AcGLK2 involves anthocyanin biosynthesis via bidirectionally modulating expression of MYB transcription factors in <i>Actinidia chinensis</i>.

Horticulture research·2026
Same journal

LpCbDR1 regulates leaf senescence and drought tolerance by activating the chlorophyll b reductase gene and stress-related genes in perennial ryegrass.

Horticulture research·2026
Same journal

T2T reference genome assembly provides insights into anthocyanin accumulation in broccoli.

Horticulture research·2026
查看所有相关文章

相关实验视频

Updated: Jul 16, 2025

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

1.2K

在西瓜中开发一个高效的CGBE基础编辑器.

Dong Wang1, Yani Chen1, Tao Zhu1

  • 1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Horticulture research
|September 18, 2023
PubMed
概括
此摘要是机器生成的。

科学家们为西瓜开发了一种新的基编辑器 (SCGBE2.0),可以实现精确的C-to-G和C-to-A突变. 该工具增强了分子育种,以改善作物特征和进化研究.

更多相关视频

Computation of Atmospheric Concentrations of Molecular Clusters from ab initio Thermochemistry
12:11

Computation of Atmospheric Concentrations of Molecular Clusters from ab initio Thermochemistry

Published on: April 8, 2020

8.2K
Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.5K

相关实验视频

Last Updated: Jul 16, 2025

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

1.2K
Computation of Atmospheric Concentrations of Molecular Clusters from ab initio Thermochemistry
12:11

Computation of Atmospheric Concentrations of Molecular Clusters from ab initio Thermochemistry

Published on: April 8, 2020

8.2K
Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.5K

科学领域:

  • 植物分子生物学 植物分子生物学
  • 基因组编辑技术的技术.
  • 提高作物质量 提高作物质量

背景情况:

  • 细胞因子和腺基编辑器 (CBE和ABE) 是一种先进的基因组编辑工具,用于修改植物中的单核酸多态 (SNP).
  • 传统的基准编辑器仅限于C-to-T和A-to-G的替换,限制了它们在作物育种中的应用.
  • 扩大基准编辑能力对于精确的基因改造至关重要,在农业上重要的物种,如西瓜.

研究的目的:

  • 为西瓜开发一种高效的基编辑器,能够诱导C-to-G和C-to-A突变.
  • 在稳定转换的西瓜植物中评估新编辑器的编辑效率和网站特异性.
  • 提供一种通用工具,用于精确基因修饰和水的局部定向和突变.

主要方法:

  • 通过修改人类A3A-CBE结构,去除UGI抑制剂并添加UNG组件,设计了一种新型的细胞因子-瓜因基编辑器 (SCGBE2.0).
  • 设计了七个特定的指导RNA (sgRNAs),针对五个关键的西瓜基因进行编辑.
  • 在稳定转换的西瓜植物中评估了SCGBE2.0的编辑效率.

主要成果:

  • 在43.2%的转基因植物中,SCGBE2.0成功诱导了C-to-G突变在C5-C9位置,最大基因转换效率为46.1%.
  • 此外,SCGBE2.0在23%的转基因植物中也在C4位置实现了C-to-A突变,达到45.9%的最大效率.
  • 编辑展示了局部偏好的突变诱导,强调了它的精度.

结论:

  • 开发的SCGBE2.0是一种高效的西瓜基编辑工具,能够产生C-to-G和C-to-A突变.
  • 这项技术提供了一种强大的方法,用于精确的基基修饰和水的局部导向和突变发生.
  • SCGBE2.0显著扩大了西瓜分子育种和遗传研究的工具包.