酸性环境可能调节干扰素-γ表达:对调节癌症和免疫细胞相互作用的含义
1Department of Biotechnology, Panjab University, Chandigarh 160014, India.
Asian biomedicine : research, reviews and news
|September 18, 2023
概括
酸性瘤微环境会损害免疫细胞的功能和相互作用. 恢复生理pH值可以提高免疫细胞对癌细胞的细胞毒性,这表明酸度是关键的障碍.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
背景情况:
- 固体瘤通常表现出酸性微环境,原因是氧气供应不佳.
- 瘤酸度可以显著改变免疫反应和细胞相互作用.
研究的目的:
- 为了研究酸性条件如何调节免疫细胞功能和癌症免疫细胞相互作用.
- 探索瘤微环境酸度对T细胞和巨细胞的影响.
主要方法:
- 培养的T细胞 (Jurkat),巨细胞 (THP-1),和HeLa细胞在不同的pH值 (7.4,6.9,6.5).
- 利用HeLa细胞条件介质 (HCM) 来模仿瘤条件的环境.
- 量化基因和蛋白质表达使用实时PCR,流细胞计和西布洛特.
主要成果:
- 酸性条件降低了T细胞和巨细胞中的干扰素玛 (IFN-γ) 的调节.
- 在酸性环境中,HeLa细胞分泌了增加的联素-18 (IL-18) 定在外体内.
- 在酸性条件下,在免疫细胞中观察到NF-κB的增强核定位.
- 当pH值正常化时,免疫细胞对HeLa细胞的细胞毒性增加.
结论:
- 酸性瘤微环境作为有效的癌症免疫细胞相互作用的重要障碍.
- 调节瘤微环境的pH值可能是增强抗癌免疫力的治疗策略.
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