为了在模型和药物发现中使用新的替代方法
Claudia Miguel Sanz1, Miriam Martinez Navarro1, Daniel Caballero Diaz1
1ZeClinics SL, IGTP (Germans Trias I Pujol Research Institute), Badalona, Spain.
Frontiers in neurology
|September 18, 2023
概括
影响全球数以百万计的人,许多患者对当前的治疗方法有抗性. 这项研究探索了新的斑马鱼模型,以改善研究和药物查,为传统方法提供了替代方案.
科学领域:
- 神经科学是一个神经科学.
- 药理学 药理学是指药理学的学科.
- 遗传学 遗传学 是一个
背景情况:
- 是一种普遍存在的神经系统疾病,影响着世界各地的不同年龄组.
- 尽管取得了进展,但超过三分之一的患者对现有的抗发作药物 (ASM) 呈现耐药性.
- 目前的ASM主要管理症状,而不是发病的潜在原因,需要改进的研究模型.
研究的目的:
- 审查目前用于研究的黄金标准动物模型.
- 探索其他模型,重点关注幼虫斑马鱼 (*ex vivo*, *in vitro*, *in vivo*).
- 突出这些模型在推进研究和药物发现中的3R (替代,减少,改进) 的作用.
主要方法:
- 已建立的哺乳动物模型的概述.
- 针对的幼虫斑马鱼模型的详细审查.
- 用于疾病建模和药物测试的药理学,遗传学和基于CRISPR/Cas9的基因编辑技术的描述.
主要成果:
- 哺乳动物模型虽然具有信息性,但在成本,吞吐量和药物查的监管批准方面存在局限性.
- 幼虫斑马鱼模型为研究提供了一种高吞吐量,成本效益和道德优势的替代方案.
- 克里斯普尔/Cas9技术显著提高了在斑马鱼中快速,高通量模型和抗药物查的潜力.
结论:
- 新的研究模型,特别是幼虫斑马鱼,对于克服当前研究的局限性至关重要.
- 这些模型有助于更深入地了解发育和开发更有效的疗法.
- 基因编辑和斑马鱼研究的进步有望加速治疗和药物开发的进展.
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