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SEPTIN3通过激活Wnt途径促进三阴性乳腺癌的进展

Guo-Zhou Wang¹, Li-Hua Yang¹, Chao Gao²

¹Department of Breast Tumor Surgery, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi, Hubei Province, 435000, People's Republic of China.

International journal of general medicine

|September 18, 2023

概括

神经元特异性septin-3 (SEPTIN3) 在三阴性乳腺癌 (TNBC) 中充当瘤基因. 抑制SEPTIN3通过激活Wnt信号通路来加速瘤生长和转移,这表明SEPTIN3是潜在的治疗标.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
癌症研究癌症研究

背景情况:

三阴性乳腺癌 (TNBC) 缺乏向疗法,导致需要新的治疗点.
神经元特异性septin-3 (SEPTIN3) 的高表达与TNBC患者的预后不佳有关.
这项研究研究了SEPTIN3在TNBC进展中的作用和调节.

研究的目的:

为了确定TNBC组织和细胞系中SEPTIN3的表达水平.
研究SEPTIN3调制 (过度表达和淘汰) 对TNBC细胞行为的功能影响.
阐明 SEPTIN3 影响 TNBC 攻击性的潜在分子机制.

主要方法:

使用定量实时PCR (qRT-PCR) 和西式涂抹来评估SEPTIN3表达.
使用lentiviral载体生成具有SEPTIN3过度表达或淘汰的TNBC细胞系.
进行了功能性测试 (CCK8,殖民地形成,划痕,透孔) 和体内瘤发生性研究.

主要成果:

在TNBC组织和细胞系中,SEPTIN3的表达显著上调.
通过SEPTIN3的敲除,抑制了TNBC细胞的增殖,入侵和迁移.
过度表达SEPTIN3促进了细胞生长,体内瘤性和侵略性,由Wnt通路激活介导.

结论:

在三阴性乳腺癌中,SEPTIN3 作为瘤基因起作用.
通过激活Wnt信号通路,SEPTIN3促进瘤的进展和攻击性.
向SEPTIN3可能为TNBC提供一种新的治疗策略.

关键词:

七月三日七月三日没有信号通路.这是入侵的入侵.它们的扩散和扩散.三重阴性乳腺癌是什么

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