相关实验视频

Updated: Jul 16, 2025

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

Published on: June 25, 2015

通过用ASFV候选免疫原体进行鼻腔和肌内注射引起的粘膜和细胞免疫反应

Lulu Xu^1,2,3, Fei Hao^2,3,4, Dae Gwin Jeong⁵

¹Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

Frontiers in immunology

|September 18, 2023

概括

这项研究确定了关键的非洲猪瘟病毒 (ASFV) 抗原,并开发了亚单位疫苗. 这两种疫苗配方都有效地刺激了猪的幽默,粘膜和细胞免疫反应.

科学领域:

兽医免疫学兽医免疫学
病毒学病毒学
生物技术是生物技术.

背景情况:

非洲猪瘟 (ASF) 由于其高传染性和死亡率,对猪业构成重大全球威胁.
鉴定疫苗开发的有效抗原对于控制ASFV至关重要.
之前的研究重点是了解ASFV复杂的抗原特征.

研究的目的:

用质谱和免疫学测试来识别新的非洲猪瘟病毒 (ASFV) 抗原.
开发和评估基于选定的ASFV抗原的子单位疫苗.
评估两种不同亚单元疫苗配方在猪中的免疫性.

主要方法:

质谱法用于从受感染的猪样本中识别ASFV抗原.
根据血清学反应和文献审查,选出了七种候选抗原.
准备了两种含有不同辅助剂的亚单位疫苗配方,并通过鼻内,肌内或联合途径给猪注射.
使用流细胞计,抗体测定 (IgG,sIgA) 和细胞因子表达分析,全面评估了幽默,粘膜和细胞免疫反应.

主要成果:

使用ASFV阳性和康复猪血清分别检测到6个和8个抗原.
七个候选抗原被选择用于疫苗开发,包括p22,p10,p30,p54,B602L,CD2V-N和F317L.
这两种疫苗组都成功地在猪中引起了显著的幽默,粘膜和细胞免疫反应.
不同的免疫路线和辅助剂影响了免疫反应的类型和程度.

结论:

已识别的ASFV抗原是子单位疫苗开发的有希望的候选人.
开发的子单位疫苗显示出显著的免疫性,刺激全面的免疫反应.
这些发现为进一步研究ASFV挑战保护和病原学研究提供了基础.

关键词:

在 ASFV 康复的猪血清中.非洲猪瘟病毒 (ASFV) 病毒免疫性评估免疫性评估质谱测量识别识别质谱测量识别再组合蛋白的表达方式.

更多相关视频

Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo

Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo

Published on: September 28, 2022

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits Oryctolagus cuniculus

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits Oryctolagus cuniculus

Published on: July 31, 2021

相关概念视频

Vaccinations

Vaccinations

Immune Response Against Viral Pathogens

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...