CircRHOT1通过表观遗传调节GPX4来限制胃癌细胞铁亡
Huan Wang1, Daniel Adam Breadner2, Ke Deng3
1Department of Medical Oncology, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao, China.
Journal of gastrointestinal oncology
|September 18, 2023
概括
循环RNAHOX转录1 (circRHOT1) 通过抑制铁亡,促进胃癌 (GC) 的进展. circRHOT1针对KAT5启动GPX4转录,使其成为GC治疗的潜在治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 胃癌 (GC) 对人类健康构成重大威胁,晚期的预后不佳.
- 确定分子机制和治疗点对于推进GC治疗至关重要.
- 循环RNAs (circRNAs) 越来越多地被认为是癌症发展和进展的关键调节者.
研究的目的:
- 研究circRHOT1在胃癌的发展和进展中的功能作用.
- 阐明 circRHOT1 参与 GC 的分子机制.
- 评估circRHOT1作为胃癌的潜在治疗点.
主要方法:
- 定量实时PCR检测GC组织和细胞系中的circRHOT1表达.
- 细胞增殖试验 (CCK-8,殖民地形成) 和异种移植瘤生长实验.
- 对铁灭菌标记物的评估,对SLC7A11和GPX4的西部斑块,以及对GPX4表观遗传调节的ChIP测定.
主要成果:
- 与非瘤组织相比,GC瘤中circRHOT1表达升高.
- Knockdown 的 circRHOT1 抑制 GC 细胞的增殖,并诱导铁亡.
- circRHOT1招募KAT5,在GPX4基因上促进H3K27Ac,从而增强其转录.
结论:
- circRHOT1通过KAT5招募促进GPX4转录,促进GC进展并抑制铁亡.
- circRHOT1代表了胃癌治疗的有前途的治疗标.
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