AQP4独立的TRPV4调制等离子体膜的水透性
Barbara Barile1, Maria Grazia Mola1, Francesco Formaggio2
1Department of Bioscience, Biotechnology and Environment, University of Bari Aldo Moro, Bari, Italy.
Frontiers in cellular neuroscience
|September 18, 2023
概括
瞬态受体潜在化物成员-4 (TRPV4) 通道促进水的运输通过细胞膜独立于水-4 (AQP4). 这种TRPV4介导的水透性在中枢神经系统细胞中提供了潜在的保护机制,防止中枢神经系统细胞的透应激.
科学领域:
- 细胞生理学 细胞生理学
- 膜运输 运输 膜运输
- 神经科学是一个神经科学.
背景情况:
- 水-4 (AQP4) 在膜外的水流中起着重要作用,特别是在中枢神经系统 (CNS) 中.
- 通道短暂受体潜在化物成员-4 (TRPV4) 涉及细胞体积调节和水运输,并提出与AQP4.4的功能合.
研究的目的:
- 独立于AQP4.4,研究TRPV4在调节血水透性的特定作用.
- 描述TRPV4依赖的水上运输的生物物理特性.
主要方法:
- 在缺乏AQP4的 (Aqp4-/-) 天体细胞中进行光灭水运输实验.
- 在过度表达TRPV4.4的HEK-293细胞中进行了素灭实验.
- 停止流光散射试验用于测量透系数 (Pf) 和激活能量 (Ea).
主要成果:
- 在缺乏AQP4的星体细胞中,TRPV4激活显著增加了细胞胀率.
- 在HEK-293细胞中过度表达TRPV4,在化学和热激活时增强了胀动力学.
- 与AQP4不同的是,TRPV4介导的水上运输表现出较低的Pf,但与促进扩散相容的Ea值,与AQP4不同.
结论:
- TRPV4提供了一条独立于AQP4的途径,通过等离子体膜透水.
- 取决于TRPV4的水上运输是一种促进的过程,与简单的扩散不同.
- TRPV4可能为水的透性提供微调机制,可能保护细胞免受透性挑战.
相关概念视频
Aquaporins
4.9K
Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.
4.9K
Regulation of Water Intake
560
Osmolality refers to the number of solute particles per kilogram of solvent in a solution. Plasma osmolality specifically indicates the total number of solute particles per kilogram of water in blood plasma. This value reflects the body's hydration status and is tightly regulated through mechanisms controlling water intake and output. While water consumption is a conscious decision, the body has intrinsic regulatory systems to maintain fluid balance. Dehydration, a state of water deficit...
560
Regulation of Water Output
335
The human body predominantly expels water through the urinary system. On average, an individual generates around 1.5 liters of urine each day. This amount can fluctuate based on how well a person is hydrated, but a critical minimum quantity of urine must be produced to ensure the body's proper functioning. Daily, the kidneys remove 600 to 1200 milliosmoles of dissolved substances, effectively excreting excess minerals and water-soluble toxins such as creatinine, urea, and uric acid from the...
335
Reabsorption and Secretion in the DCT and Collecting Duct
1.2K
The early phase of the DCT manages the reabsorption of approximately 10-15% of filtered water, 5–10% of filtered sodium, and 5–10% of filtered chloride. This process is facilitated by Na+–Cl− symporters in apical membranes and sodium-potassium pumps, as well as Cl− leakage channels in basolateral membranes. The early DCT also stands out as a site where parathyroid hormone (PTH) stimulates calcium reabsorption, depending on the body's requirements.
The distal...
The distal...
1.2K
Membrane Fluidity
11.3K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
11.3K
Membrane Asymmetry Regulating Transporters
4.6K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
4.6K


