相关实验视频

Updated: Jul 16, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

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在CRISPR/Cas9中介的Caenorhabditis nigoni基因组编辑中,使用保存的dpy-10共同转换标记物进行了编辑

Charlotte P Choi1, Anne M Villeneuve1

  • 1Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305 U.S.A.

microPublication biology
|September 18, 2023
PubMed
概括

研究人员使用dpy-10基因创建了一个新的标记物,可以轻松追踪线虫Caenorhabditis nigoni中的CRISPR/Cas9基因编辑. 这个工具简化了在这个新兴模型生物体中制造和发现遗传变化的过程.

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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

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CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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