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人类胚胎膜中中介细胞的异质性在满期时

Salvador Cortes-Sandoval^1,2, Vicente Seco-Rovira², Ester Beltrán-Frutos²

¹Department of Obstetrics and Gynecology, Virgen de la Arrixaca Hospital, IMIB, Murcia, Spain.

Histology and histopathology

|September 18, 2023

概括

人类胚胎膜 (hAM) 含有异构的介质细胞. 一些细胞表现出衰老的纤维细胞表型与纤维内素积累,影响再生医学应用.

科学领域:

组织生物学组织生物学
再生医学是一种再生医学.
细胞异质性细胞异质性

背景情况:

人类羊水膜 (hAM) 有医疗应用.
可以培养和分化hAM中介质细胞.
之前的研究表明间细胞异质性.

研究的目的:

为了研究hAM介质细胞*in situ*的细胞异质性.
为了表征hAM纤维细胞层内的介质细胞类型.

主要方法:

对hAM样本的组织学和组织化学分析.
光和传输电子显微镜 (TEM).
细胞群的半定量分析.

主要成果:

显著的介质细胞类型I-III和霍夫尔细胞.
在细胞类型中观察到对PAS,维门丁,纤维素,康卡纳瓦林-A,VGEF,TGF-β2,PDGF-C和FGF-2的阳性增加.
第二种类型的细胞占总体的60%;TEM揭示了一种中间的II-III类型,在第三种类型的细胞中具有RER扩张和纤维素积累.

结论:

在hAM纤维细胞层中的介质细胞是异质的.
一些细胞表现出不分化的表型,而另一些表现出老化的纤维细胞表型与纤维素积累.
这些发现与用于再生医学的hAM细胞提取以及理解胎儿膜破裂有关.

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