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Merlin Dähmcke¹, Martin Busch¹, Johanna M Pfeil¹

¹Department of Ophthalmology, University Medicine Greifswald, Greifswald, Germany.

Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift fur Augenheilkunde

|September 18, 2023

概括

血清和玻璃体中的特定microRNAs (miRNAs) 在患有血管相关视网膜疾病的患者中显示出改变的表达,表明它们作为诊断生物标记物的潜力. 结合多个miRNA可能会提高与年龄相关的黄斑变性和糖尿病视网膜病变等疾病的预测准确性.

科学领域:

眼科医生眼科眼科
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

与血管相关的视网膜疾病会导致显著的视力丧失.
生物标志物识别对于疾病管理和向治疗至关重要.
微RNAs (miRNAs) 调节基因表达,可以作为疾病指标.

研究的目的:

在患有视网膜疾病的患者的血清和玻璃体中调查特定miRNA的存在.
为了确定这些miRNA是否可以作为无细胞循环生物标志物.
评估与年龄相关的黄斑变性 (AMD) 和糖尿病视网膜病变 (DR) 的诊断潜力.

主要方法:

定量实时聚合酶连锁反应 (qPCR) 用于miRNA分析.
视网膜疾病患者和对照组之间的表达水平进行了比较.
使用接收器操作特征 (ROC) 分析来评估诊断准确性.

主要成果:

在新血管AMD中增加了miR-29a-3p和miR-192-5p的血清表达.
在增殖性DR中,mIR-335-5p,mIR-192-5p和mIR-194-5p的血清水平升高.
在增殖性DR中减少了miR-100-5p的玻璃体表达.

结论:

研究的miRNA显示出与血管相关的视网膜疾病的生物标志物的潜力.
特定的miRNA配置文件在疾病状态和对照组之间有所不同.
结合多个miRNA可能会增强诊断和预测能力.

关键词:

与年龄有关的黄斑变性.生物标志物生物标志物糖尿病视网膜病变 - 糖尿病视网膜病变微RNAs是一种微RNA.视网膜静脉封闭视网膜静脉封闭

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...