在Candida albicans复合体的 vulvovaginal分离物中生物化学类型和病原性评估
Soraya Morales-López1, Keiner Ustate2, Zulay Pedrozo3
1Grupo CINBIOS, Programa de Microbiología, Universidad Popular del Cesar, Valledupar, Colombia; Laboratorios Nancy Flórez García S.A.S., Valledupar, Colombia. sorayamorales@unicesar.edu.co.
Biomedica : revista del Instituto Nacional de Salud
|September 18, 2023
概括
这项研究对Candida albicans复杂分离物的特征进行了鉴定,发现了可变的表型特征和致病因素. 准确的物种识别需要超越基本测试的先进方法.
科学领域:
- 医学真菌学 医学真菌学
- 微生物学 微生物学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 坎迪达阿尔比坎斯复合体包括密切相关的物种,如C. albicans,C. dubliniensis和C. africana.
- 准确识别这些物种对于理解它们独特的临床行为至关重要.
研究的目的:
- 通过表型和病理学来表征一种Candida albicans复杂分离的集合.
- 评估用于识别这些真菌病原体的标准和新方法.
主要方法:
- 使用的CHROMagarTM Candida,生殖管测试 (三种方法),克拉米多菌形成,以及在手工 (迷香,烟草) 上的殖民地形态.
- 采用MALDI-TOF作为识别的黄金标准.
- 评估的致病性因素包括血液溶解活性,凝血酶产生,生物膜形成和与黄金葡萄球菌的共同培养.
主要成果:
- MALDI-TOF证实所有300个分离物都是Candida albicans的.
- 在生殖管生产 (43.7%),克拉米多胞形成 (47%) 和手工的殖民地颜色 (6%棕色的迷香,5%的烟草) 上观察到可变的结果.
- 在与血液 (50%) 结合的土豆德克斯特酸盐上观察到显著的溶血活性,而凝固酶的产生是普遍的,生物膜的形成有所不同.
结论:
- 标准的表型测试显示,在Candida albicans复合体内区分物种方面存在局限性.
- 先进的技术,如蛋白质组学或分子分析是必要的,以确定物种识别.
- MALDI-TOF有效地识别了C. albicans,但不能在复合体内区分密切相关的物种.
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