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线粒体功能障碍和氧化应激有助于丹尼奥河流中子烯的跨代毒性

Jordan S Kozal¹, Nishad Jayasundara¹, Andrey Massarsky¹

¹Nicholas School of the Environment, Duke University, Durham, NC, USA.

Aquatic toxicology (Amsterdam, Netherlands)

|September 18, 2023

概括

母亲暴露于多环芳化合物 (PAH) 的子烯 (BaP),导致斑马鱼后代的线粒体功能障碍. 这些影响,包括呼吸障碍和DNA损伤,发生在几代人之间,突出显示了跨世代毒性风险.

科学领域:

环境毒理学环境毒理学
线粒体生物学线粒体生物学
发展生物学发展生物学

背景情况:

多环芳 (PAH) 由于潜在的跨代影响,对人类和野生动物的健康构成风险.
线粒体对细胞能量至关重要,是从母亲遗传的,易受毒素的影响.
甲 (BaP) 是一种PAH模型,也是已知的线粒体毒素.

研究的目的:

调查线粒体在本佐甲烯 (BaP) 跨代毒性中的作用.
评估母体BAP暴露对斑马鱼后代线粒体功能和健康的影响.

主要方法:

成熟的雌性斑马鱼 (F0) 在21天内暴露于不同剂量的BaP (12.5,125,1250μg/g饮食) 中.
F0雌性与未暴露的雄性进行繁殖;由此产生的胚胎 (F1) 分析了线粒体功能,DNA完整性和氧化应激.
在体内氧气消耗率 (OCRs) 被测量以评估代谢分割和线粒体呼吸.

主要成果:

母体暴露的F1胚胎显示基底和线粒体呼吸减少,ATP周转率改变,质子泄漏增加,储备能力减少.
在后代中观察到线粒体DNA (mtDNA) 拷贝数的显著减少和mtDNA损伤的增加.
后代表现出改变的氧化应激生物标志物和游泳低活性,即使在12.5μg BaP/g饮食的最低观察效应水平 (LOEL) 中也可以看到效应.

结论:

母亲暴露于BaP会诱导斑马鱼后代显著的线粒体损伤.
后代的线粒体功能障碍可以在没有明显影响的情况下在母亲的卵巢中表现出来,这表明了跨代的影响.
这些发现凸显了PAH诱导的线粒体毒性对后代产生影响的潜力.

关键词:

子烯 (a) 烯 (Benzo) 是一种跨世代的毒性跨世代的毒性线粒体中的线粒体.氧化应激是一种氧化应激.多环芳香碳化合物多环芳香碳化合物斑马鱼是一种斑马鱼.

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