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相关概念视频

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

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Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for...
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High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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相关实验视频

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Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis
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基于程序控制的脉冲发光二极管的多波长深紫外线吸收探测器.

Ibraam E Mikhail1, Mohamed Hemida1, Leo Lebanov1

  • 1Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences (Chemistry), University of Tasmania, Hobart, Tasmania 7001, Australia.

Journal of chromatography. A
|September 18, 2023
PubMed
概括

一个新的脉冲多波长紫外线吸收探测器 (PMLAD) 是使用单板计算机和FPGA开发的. 这种新的系统能够高精度地同时分析像,咖啡因和阿司匹林这样的药物.

关键词:
深紫外线吸收深紫外线的吸收高性能液体色谱学 高性能液体色谱学多波长检测的多波长检测.脉冲发光二极管是一种脉冲发光二极管.红色皮塔亚 (Pitaya) 是一个红色的皮塔亚.

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科学领域:

  • 分析化学 分析化学
  • 频谱学是一种光谱学.
  • 仪器化 仪器化 仪器化

背景情况:

  • 传统的紫外线吸收检测方法在速度和复杂化能力方面可能受到限制.
  • 深紫外发光二极管 (DUV-LED) 提供了对紧和高效的吸收度测量的潜力.
  • 集成先进的计算与光学检测对于现代分析仪器来说至关重要.

研究的目的:

  • 引入一种新型的脉冲多波长紫外线吸收探测器 (PMLAD),以提高分析性能.
  • 通过液态染色学来证明该系统在使用液体染色学同时进行制药分析的能力.
  • 为了验证探测器的线性,准确性和定量限制.

主要方法:

  • 使用带有现场可编程网关阵列 (FPGA) 的单板电脑 (SBC) 为三个DUV-LED (235,250,280nm) 产生同步的微脉冲.
  • 数据采集和处理是使用一个定制的程序进行的,通过光纤传输到流细胞的联合DUV-LED输出.
  • 一个超快的光二极管记录了DUV脉冲强度,该系统与标准的液态色谱仪相结合.

主要成果:

  • PMLAD表现出极好的线性,线性的上限 (A95%) 从1768到2189mAU,有效路径长度≥98.0%.
  • 流浪光的测量结果为≤0.9%.
  • 实现了对,咖啡因和阿司匹林的同时确定,具有高线性 (r2 ≥ 0.99996) 和低量化极限 (LOQs) 到0.10μg/mL.

结论:

  • 开发的脉冲多LED吸收探测器 (PMLAD) 为制药分析提供了一种敏感和选择性的方法.
  • 波长之间的吸收比率的使用有助于化合物识别.
  • 这种新的方法为染色学中多波长紫外线检测提供了一个强大的平台.