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下游生物处理工具箱用于治疗性病毒载体

Ryan Kilgore¹, Arianna Minzoni¹, Shriarjun Shastry²

¹Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, United States.

Journal of chromatography. A

|September 18, 2023

概括

下游加工的进步对于在医学和生物技术中广泛使用病毒载体至关重要. 净化技术的创新确保了这些重要治疗剂的高产量,纯度和安全性.

科学领域:

生物技术和生物制造
基因治疗和病毒载体技术
下游开发过程下游开发过程

背景情况:

病毒载体是基因疗法,疫苗和农业应用的重要工具.
越来越多的临床和商业需求需要可扩展和具有成本效益的生物制造策略.
目前的下游加工方法是从蛋白质净化中改进的,需要针对病毒载体进行专门的优化.

研究的目的:

为在过去十年中开发的病毒载体下游生物工艺工具箱提供全面的概述.
讨论病毒载体净化当前的创新和未来的方向.
突出下游加工在使基于病毒载体的药物的成功中发挥的关键作用.

主要方法:

对各种病毒载体类型 (例如,腺相关病毒,晶状病毒,腺病毒) 的近期亲缘关系连接体发现进展的综述.
探索新的吸附材料和格式,包括膜和3D打印单体.
综合工艺设计的分析,将新的配体和吸附剂与高级分析方法相结合,用于质量属性监测.

主要成果:

根据病毒载体的独特特征量身定制的净化工具箱的显著扩展.
开发创新的配体和吸附剂,使得有效的捕获和净化.
复杂的工艺布局和分析技术的出现,以确保产品质量,有效性和安全性.

结论:

下游工艺技术是病毒载体临床和商业成功的关键推动因素.
在配体,吸附剂,工艺设计和分析方面的持续创新对于满足未来需求至关重要.
下游生物处理工具箱的演变支持病毒载体作为一类有希望的生物药物的进步.

关键词:

在 AAV AAV AAV 中.吸附剂吸附剂是一种吸附剂.亲和关系连接体的亲和关系连接体.染色体学染色体学是一种染色学.基因治疗是一种基因疗法.病毒载体病毒载体

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