结合ATP的卡塞特蛋白ABCC8促进草水果中安托西亚尼的积累
Ting Qian1, Xiaoshan Wang1, Jingjing Liu1
1School of Horticulture, Anhui Agricultural University, Hefei, 230036, China.
Plant physiology and biochemistry : PPB
|September 18, 2023
概括
草水果的质量与酸有关. 研究人员确定了ABCC8基因,该基因对于将这些化合物输送到真空细胞至关重要,并发现其表达受到特定的转录因子的调节.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 生物化学 生物化学
背景情况:
- 安西是重要的黄化合物,影响水果质量和健康益处.
- 虽然人们已经了解了酸生物合成,但草果中的运输机制在很大程度上是未知的.
研究的目的:
- 为了研究草果中特定的ATP结合盒 (ABC) 载体基因ABCC8的功能.
- 阐明控制ABCC8表达的调节机制及其在素积累中的作用.
主要方法:
- 从草中分离和描述FvABCC8基因.
- 定量逆转录PCR (qRT-PCR) 用于分析成熟期间的FvABCC8转录水平.
- 草果中的过度表达和RNA干扰 (RNAi) 的过渡性.
- 在Arabidopsis中使用MYB和bHLH转录因子进行促进体活性测定.
- 分析糖和酸 (ABA) 对FvABCC8功能的影响.
主要成果:
- 在草果成熟期间,FvABCC8表达与素积累有很强的相关性.
- 过度表达FvABCC8增加了安东素含量,而RNAi减少了它.
- 该ABCC8促进体被转录因子MYB10,bHLH33和MYC1.1激活.
- 糖在FvABCC8-过度表达的阿拉比多普西斯中增强了氨酸的积累,这些线路显示ABA敏感性降低.
结论:
- 草真空阳素运输很可能是由ABCC输送器FvABCC8.8介导的.
- FvABCC8的表达受到MYB10,bHLH33和MYC1转录因子的调节.
- FvABCC8在控制草果中的安托西亚宁水平方面发挥着重要作用.
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