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鉴定和功能分析的骨髓分化因子88 (MyD88) 在早期发展的Haliotis多元色彩的功能分析

Xiaoting Gong¹, Min Li¹, Lili Zhang¹

¹Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, Xiamen, 361021, China.

Fish & shellfish immunology

|September 18, 2023

概括

标识了 abalone Myeloid分化因子88 (hdMyD88) 基因,在8细胞阶段表达最高. Knockdown 实验表明 hdMyD88 在 abalone 幼虫的早期天生的免疫力中起作用.

科学领域:

海洋生物学海洋生物学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

骨髓状细胞分化因子88 (MyD88) 是托尔类受体 (TLR) 信号传输中的一个关键适应蛋白.
了解MyD88在海等无脊椎动物中的作用对于洞察天生的免疫进化至关重要.

研究的目的:

为了识别和表征MyD88基因 (hdMyD88) 在Haliotis多彩 (abalone).
研究hdMyD88在幼虫早期发育过程中的表达模式和功能作用.

主要方法:

基因克隆和测序以确定hdMyD88.8的全长cDNA.
定量PCR (qPCR) 分析在不同发育阶段的hdMyD88 mRNA表达水平.
使用dsRNA的RNA干扰 (RNAi) 来评估hdMyD88淘汰的功能影响.
整体胚胎在现场杂交以定位hdMyD88表达.

主要成果:

hdMyD88基因具有1927 bp的cDNA编码437个氨基酸,具有保存死亡和TIR域.
在整个早期发育过程中hdMyD88mRNA的构成性表达,在8细胞阶段达到峰值,在三角球阶段最低.
MyD88 knockdown显著降低了三角圈和虫幼虫中的hdMyD88mRNA水平.
hdMyD88的表达被局部化到三叶虫和虫幼虫的内脏质量.

结论:

鉴定到的hdMyD88基因具有典型的MyD88结构特征.
hdMyD88参与鱼早期的天生的免疫反应.
这些发现表明hdMyD88在对潜在病原体的免疫防御中起着重要作用.

关键词:

斑多色,MyD8888 这是一种多色斑.与免疫相关的基因幼虫幼虫幼虫是一种

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