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在Trachinotus ovatus中分子表征补充调节因子CD46及其在抗微生物免疫反应和补充调节中的作用

Meiqin Mao¹, Yinhui Peng², Kianann Tan¹

¹College of Marine Sciences, Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou, 535011, China.

Fish & shellfish immunology

|September 18, 2023

概括

黄金的新CD46基因ToCD46调节补充系统,并对抗细菌感染. 这一发现提供了关于鱼类免疫力和补充系统进化的见解.

科学领域:

免疫学免疫学免疫学
海洋生物学海洋生物学
遗传学是一个遗传学.

背景情况:

CD46是补体系统的关键调节者,作为一种参与自和早期感染控制的病原体受体.
黄金 (Trachinotus ovatus) 是一个重要的水产养殖物种,但其免疫机制,特别是涉及CD46,尚未完全理解.

研究的目的:

在金色的庞巴诺中识别和描述一种新的CD46基因 (ToCD46).
调查ToCD46在金色庞巴诺的免疫反应中的表达模式,功能角色和进化意义.

主要方法:

基因鉴定和序列同质性分析,将ToCD46与其他物种进行比较.
在基底条件下和细菌挑战 (Streptococcus agalactiae,Vibrio alginolyticus) 后组织表达概况.
功能性检测包括血解活性,细胞灭亡抑制和体外抗菌实验.

主要成果:

与哺乳动物CD46.6相比,ToCD46表现出与teleost CD46具有更高的序列相似性.
ToCD46被广泛表达,在肝脏和头的水平最高,在感染后的免疫组织上调节.
重组ToCD46 (rToCD46) 降低了血清溶血活性,保护细胞免受损伤,并显示了对V. alginolyticus的抗菌活性.

结论:

图CD46在黄金庞巴诺的先天免疫反应中发挥着重要作用,特别是在调节补充剂激活和对抗阴性细菌感染方面.
这些发现提供了基本数据,以了解金色庞巴诺的补体系统的演变,并制定鱼类抗病的策略.

关键词:

具有抗菌活性,具有抗菌活性.CD46 CD46 CD46 CD46 CD46 CD46 CD46 CD46 CD46 CD46 CD46 CD46 CD46 CD46 CD46 CD46 CD46 细胞亡是细胞的亡.血液溶解血液溶解组织表达的表达方式它们中的一个是Trachinotus ovatus.

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