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Updated: Jul 16, 2025

V3 Stain-free Workflow for a Practical, Convenient, and Reliable Total Protein Loading Control in Western Blotting
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一种基于光Ponceau S的总蛋白质正常化方法,用于常规和具有挑战性的免疫块样本

Claire Verzeroli1, Charlotte A Hernandez1, Fabien Zoulim1

  • 1Hepatitis Viruses and Pathobiology of Chronic Liver Diseases - LabEx DEVweCAN, Inserm U1052, Cancer Research Centre of Lyon, F-69003, Lyon, France; University of Lyon, F-69003, Lyon, France; University of Lyon 1, ISPB, Lyon, F-69622, France; CNRS UMR5286, F-69083, Lyon, France; Centre Léon Bérard, F-69008, Lyon, France.

Analytical biochemistry
|September 18, 2023
PubMed
概括

整条车道染色促进了免疫阻塞的正常化. 一种新的光Ponceau S方法提供了一种灵敏,可重复和具有成本效益的方法,可以在各种样本中使蛋白质检测正常化.

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相关概念视频

Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
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