Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Early Endosome: Endocytosis of Transferrin01:28

The Early Endosome: Endocytosis of Transferrin

3.3K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
3.3K
ER Retrieval Pathway01:45

ER Retrieval Pathway

3.9K
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
3.9K
Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

2.7K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
2.7K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

3.6K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.6K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

8.6K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Pathomechanism of Fever-Induced Liver Failure in NBAS Deficiency and Treatment Effect of NAC-Observations In Vitro and In Vivo.

Liver international : official journal of the International Association for the Study of the Liver·2026
Same author

Sleep deprivation increases levels of the synaptic density marker SV2A in the human brain.

PLoS biology·2026
Same author

Conduct problems and callous-unemotional traits in pre-schoolers: Psychosocial profiles in a Brazilian birth cohort.

Journal of child psychology and psychiatry, and allied disciplines·2026
Same author

Single-Dose Creatine Reduces Sleep Deprivation-Induced Deterioration in Cognitive Performance.

Nutrients·2026
Same author

Social Information Processing and Aggressive Behavior in Early Childhood: A Brazilian Birth Cohort Study.

Journal of interpersonal violence·2026
Same author

Quantifying and visualising heterogeneity in cumulative adverse childhood experiences scores: A national survey of US children.

Preventive medicine·2026

相关实验视频

Updated: Jul 16, 2025

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells
08:53

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

Published on: May 16, 2017

8.7K

NECAB2是一种内体蛋白质,对状功能很重要.

Diones Bueno1, Partha Narayan Dey1, Teresa Schacht1

  • 1University Medical Center of the Johannes Gutenberg-University Mainz, Institute for Molecular Medicine, Germany.

Free radical biology & medicine
|September 18, 2023
PubMed
概括

神经元结合蛋白2 (NECAB2) 对于条状细胞功能和线粒体健康至关重要. 它的缺失会损害线粒体功能,导致氧化应激和纹状体中的突触损失.

更多相关视频

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons
06:18

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

Published on: April 3, 2014

13.0K
An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
13:46

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons

Published on: July 11, 2020

5.8K

相关实验视频

Last Updated: Jul 16, 2025

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells
08:53

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

Published on: May 16, 2017

8.7K
Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons
06:18

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

Published on: April 3, 2014

13.0K
An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
13:46

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons

Published on: July 11, 2020

5.8K

科学领域:

  • 神经科学是一个神经科学.
  • 细胞生物学 细胞生物学
  • 线粒体生物学 线粒体生物学

背景情况:

  • 突触功能依赖于线粒体的ATP生产.
  • 线粒体功能障碍破坏细胞的氧化还原平衡,促进氧化应激.
  • 条形体的复杂连接性使其易受线粒体损伤的影响.

研究的目的:

  • 调查神经元结合蛋白2 (NECAB2) 在条状细胞功能和线粒体平衡中的作用.
  • 为了阐明NECAB2缺乏对线粒体动力学和线条体内的氧化还原平衡的影响.

主要方法:

  • 使用了全球Necab2淘汰赛小鼠模型.
  • 分析了线粒体形态,功能和氧化还原状态 (超氧化物,谷,DNA氧化).
  • 在氧化应激下,在条状培养物中检查了内分体-线粒体相互作用.

主要成果:

  • Necab2淘汰赛小鼠表现出氧化应激标志物 (超氧化物,DNA氧化) 的增加和谷氨的减少.
  • 在Necab2的淘汰赛条纹中,线粒体较小,数量更多,并且显示出缺余能力受损.
  • 在Necab2淘汰赛小鼠中观察到纹状突触的丧失和行为缺陷 (动机减少,感官门的改变).

结论:

  • NECAB2对于维护线粒体平衡和排列体中氧化还原平衡至关重要.
  • 缺少NECAB2导致线粒体功能障碍,氧化应激和突触损失,导致条状功能障碍.
  • NECAB2可能会调解涉及线粒体应激反应的内体内体内内体内体内途径.