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哺乳动物的气味结合蛋白容易形成无形聚合物和粉样纤维

Olga V Stepanenko¹, Anna I Sulatskaya¹, Maksim I Sulatsky²

¹Laboratory of Structural Dynamics, Stability and folding of Proteins, Institute of Cytology of the Russian Academy of Sciences, 4 Tikhoretsky ave, 194064 St. Petersburg, Russia.

International journal of biological macromolecules

|September 18, 2023

概括

牛臭剂结合蛋白 (bOBP) 在压力下形成聚合物,损害了连接物结合. 在特定的条件下,具有细胞毒性的粉样纤维素出现,影响嗅觉蛋白质功能.

科学领域:

生物化学生物化学
结构生物学结构生物学
嗅觉受体研究嗅觉受体研究

背景情况:

气味结合蛋白 (OBPs) 对于嗅觉至关重要,它们在其结构中结合了气味.
牛臭剂结合蛋白 (bOBP) 作为研究OBP结构动态和连接体相互作用的模型.

研究的目的:

调查BOBP的结构组织.
在不同的体外条件下检查bOBP的联结能力.
为了确定影响bOBP聚合和潜在的粉样蛋白形成的因素.

主要方法:

在体外分析牛臭剂结合蛋白 (bOBP) 的结构和聚合.
在生理和压力条件下 (温度,pH,,化学处理) 进行带结合试验.
超分子复合体和纤维状聚合物的表征.

主要成果:

在生理条件下,bOBP在不损害单体完整性的情况下自然形成寡合体和小聚合物.
升高的温度和pH值促进更大,更密集的复合体,降低了连接体结合亲和力.
动,酸化或六化醇治疗会诱导粉样纤维的形成,其特点是高细胞毒性.
纤维生成涉及单体解离,β-桶不稳定化和保存的β-链.

结论:

环境压力因素对bOBP结构和功能产生重大影响,导致结合因子的结合减少.
在特定的条件下,bOBP可以形成细胞毒性粉样纤维,这突出了对蛋白质稳定性和细胞健康的潜在影响.
了解bOBP聚合途径对于理解嗅觉蛋白质的行为和稳定性至关重要.

关键词:

无形聚合物和粉样化合物细胞毒性细胞毒性气味剂结合蛋白质气味剂结合蛋白质嗅觉功能障碍嗅觉功能障碍

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