Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

CD4<sup>+</sup> T cells impair tumor growth through IL-3 and TNF-dependent vascular damage.

Science (New York, N.Y.)·2026

Same author

CLEC14A correlates with neutrophil infiltration in hepatocellular carcinoma and mediates neutrophil recruitment across liver endothelial cells.

The Journal of pathology·2026

Same author

High-Plex Tissue Imaging with Conventional Immunofluorescence Platforms and Open-Source Software via Iterative Bleaching Extends Multiplexity (IBEX).

Methods in molecular biology (Clifton, N.J.)·2026

Same author

Agonistic anti-CD40 antibody treatment converts resident regulatory T cells into activated type 1 effectors within the tumor microenvironment.

Immunity·2026

Same author

Immunological knowledge.

Nature immunology·2026

Same author

Tumour-brain crosstalk restrains cancer immunity via a sensory-sympathetic axis.

Nature·2026

Search research articles

相关实验视频

Updated: Jul 16, 2025

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

Published on: April 22, 2014

在炎症研究中的动态多重组织成像.

Stefan Uderhardt^1,2,3, Georgiana Neag^1,2,3, Ronald N Germain^4,5

¹Department of Medicine 3-Rheumatology and Immunology, Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany.

Annual review of pathology

|September 18, 2023

概括

双光子显微镜 (2PM) 能够在体内对深层组织进行成像,揭示动态免疫细胞的行为,这对于理解炎症至关重要. 这项技术有助于将细胞动力学与复杂组织环境中的 in situ 功能和分子模式相关联.

关键词:

动态成像图像处理系统静脉内成像检查静脉内成像检查多重成像成像多重成像组织成像组织成像.两光子显微镜技术

更多相关视频

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

相关实验视频

Last Updated: Jul 16, 2025

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

Published on: April 22, 2014

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
生物医学成像技术生物医学成像技术

背景情况:

炎症涉及复杂组织环境中的动态免疫细胞相互作用.
鉴于ex vivo模型的局限性,评估这些细胞动态在体内至关重要.
深层组织成像是必要的,以观察这些过程远远低于器官表面.

研究的目的:

为了说明两光子显微镜 (2PM) 如何促进免疫细胞动态的体内研究.
为了将观察到的免疫细胞行为与它们的 in situ 功能和调节分子模式联系起来.
审查生物研究中2PM的实际方面和潜在的混因素.

主要方法:

利用与现有成像系统集成的双光子激发激光器.
在体内进行长期深层组织成像.
分析应用2PM研究免疫细胞在炎症环境中的行为.

主要成果:

2PM允许可视化免疫细胞迁移和深层组织内部的相互作用.
研究表明,免疫细胞动态和它们在现场的功能作用之间存在联系.
在体内影响免疫细胞行为的分子模式已被使用2PM阐明.

结论:

双光子显微镜是研究活体免疫细胞动态行为的强大工具.
了解局部细胞动态对于理解炎症过程至关重要.
需要仔细考虑2PM的方法,以确保可靠的科学结论.