发现主要的15-30 nt哺乳动物小RNA,它们的生物发生和功能
Hejin Lai1, Ning Feng1, Qiwei Zhai2,3
1CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.
Nature communications
|September 18, 2023
概括
哺乳动物小RNA (sRNA) 主要以2结尾.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 小RNAs (sRNAs) 是基因表达的关键调节者.
- 已知的sRNA如miRNA,tsRNA和srRNA具有3'-OH终端.
- 在15-30 nt范围内的其他sRNA类型的患病率仍然不清楚.
研究的目的:
- 为了研究哺乳动物小RNAs (sRNAs) 在15-30nt大小范围内的主导形式.
- 开发和验证具有2',3'-循环酸盐 (3'-cP) 末端 (sRNA-cPs) 和3'-OH末端 (sRNA-OHs) 的sRNA分析的新方法.
- 探索sRNA-cPs的生物发生,功能和调节作用.
主要方法:
- 开发TANT-seq用于sRNA-cPs和sRNA-OHs的同时分析.
- 使用TE-qPCR和北方斑块验证TANT-seq的验证.
- 使用血管新生素和RNase4对sRNA-cp生物发生的研究.
主要成果:
- 大约90%的哺乳动物sRNA在15-30nnt范围内表现出3'-cP终端.
- TANT-seq揭示了大量的sRNA-cP,它们通常具有与sRNA-OHs不同的序列.
- 血管新生素和RNase 4被确定为sRNA-cp生物生成中的关键酶.
- 与sRNA-OHs相比,sRNA-cP对Ago2具有更高的结合亲和力,并调节基因表达,包括snR-2-cP对Bcl2mRNA进行向以调节亡.
- sRNA-cP可以在Ago2复合体内调解目标RNA裂变,类似于miRNA,无论它们的3'-cP修饰如何.
结论:
- 大多数15-30 nt的哺乳动物sRNA都是sRNA-cP,显著扩大了已知的调节性RNA的谱系.
- TANT-seq为全面的sRNA分析提供了一个强大的工具.
- sRNA-cPs在基因调节和细胞过程中发挥着重要作用,如细胞亡,通过Ago2.
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