功能和系系学支持ASIC类的Deg/ENaC通道在Placozoa中的独立进化
Wassim Elkhatib1,2, Luis A Yanez-Guerra3, Tatiana D Mayorova4
1Department of Biology, University of Toronto Mississauga, 3359 Mississauga Road, Mississauga, ON, L5L 1C6, Canada.
Communications biology
|September 18, 2023
概括
研究人员在Trichoplax adhaerens中发现了一种新型的质子通道,TadNaC2. 这一发现表明非双边无脊椎动物中ASIC类通道的独立进化,扩大了我们对Deg/ENaC通道多样性的理解.
科学领域:
- 分子生物学分子生物学
- 进化生物学 进化生物学
- 生物物理学的生物物理.
背景情况:
- 酸感应离子通道 (ASICs) 是双边体中的质子通道,是多样化的Deg/ENaC通道家族的一部分.
- 德格/ENaC家族还包括和机械通道,突出显示了功能多样性.
- 了解这些通道的进化起源和变异对于理解不同物种的离子通道功能至关重要.
研究的目的:
- 研究非双胞胎无脊椎动物中Deg/ENaC通道的存在和特征,特别是Trichoplax adhaerens.
- 阐明在已识别的T. adhaerens通道中的质子关的独特生物物理性质和分子决定因素.
- 确定T. adhaerens Deg/ENaC通道与已知的ASIC和其他相关通道家族的系系关系.
主要方法:
- 电生理学记录以描述T. adhaerens通道 (TadNaC2) 的生物物理特性.
- 结构建模和局部定向突变发生,以确定参与质子门的关键残留物.
- 来自各种甲动物和非甲动物物种的Deg/ENaC通道序列的遗传学分析.
主要成果:
- 鉴定了TadNaC2,T. adhaerens中的一个以质子激活的Deg/ENaC通道,具有独特的特征,如速移,减少质子灵敏度和两相电流.
- TadNaC2对阿米洛里德和Ca2+离子的敏感性较低,与许多ASIC通道不同.
- 结构和遗传学分析显示,TadNaC2质子关机制与ASIC不同,T. adhaerens Deg/ENaC通道形成一个独特的类,表明独立的进化.
- 遗传学分析确定了非特征的甲动物Deg/ENaC通道,并提供了Metazoa以外的Deg/ENaC通道的证据.
结论:
- 类似ASIC的道在非双边T. adhaerens及其类Placozoa中独立演变.
- 与ASIC通道相比,TadNaC2具有独特的生物物理性质组合和与ASIC通道相比,对质子关的独特分子决定因素.
- 这项研究扩大了已知的Deg/ENaC通道的多样性,并表明它们存在于早期分支的甲基动物中,甚至可能在甲基动物之外.
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