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Updated: Jul 16, 2025

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

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基于基因组测序的转录组分析揭示了Peucedanum praeruptorum中的新基因

Cheng Song1, Yingyu Zhang2, Yunpeng Zhang3

  • 1Anhui Dabieshan Academy of Traditional Chinese Medicine, Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, Anhui Engineering Research Center for Eco-agriculture of Traditional Chinese Medicine, College of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, 237012, China. lanniao812329218@163.com.

BMC genomic data
|September 18, 2023
PubMed
概括

在Peucedanum praeruptorum Dunn中提前插入螺栓可以降低氨酸含量. 这项研究确定了参与氨酸生物合成和螺栓的关键基因,为管理这种药用草药提供了洞察力.

关键词:
库马林是一种素.高通量测序的高通量测序化是一种化过程.皮塞丹姆 (Peucedanum praeruptorum) 是一个古老的植物.转录法规 转录法规

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Published on: April 25, 2015

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Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates08:59

Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates

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Ribosome Profiling02:24

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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