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在人类单细胞和CD4⁺ T细胞中,TLR8逃脱了X染色体不活化

Ali Youness¹, Claire Cenac¹, Berenice Faz-López¹

¹Institut Toulousain des Maladies Infectieuses et Inflammatoires (INFINITY), UMR 1291 INSERM, CNRS, Hôpital Purpan, Université de Toulouse, 31024, Toulouse, France.

Biology of sex differences

|September 18, 2023

概括

收费类受体8 (TLR8) 与TLR7一样,在人类免疫细胞中逃避X染色体失活 (XCI). 这种逃逸导致女性的TLR8蛋白水平更高,可能会影响免疫反应和自身免疫疾病的风险.

科学领域:

免疫学免疫学免疫学
遗传学遗传学是一个
分子生物学分子生物学

背景情况:

收费类受体 (TLRs) 7和8对于先天免疫和自身免疫性疾病至关重要.
这些受体是由相邻的X链基因编码的.
以前已经证明TLR7可以在女性免疫细胞中逃避X染色体失活 (XCI),但TLR8的状态尚不清楚.

研究的目的:

调查TLR8是否也可以在人类免疫细胞中避开XCI.
确定TLR8 XCI逃逸是否与蛋白质表达中的性二态有关.

主要方法:

在位杂交RNA光 (RNA FISH) 来可视化TLR7和TLR8初级转录.
分析女性单细胞和T淋巴细胞,克莱因费尔特综合征 (KS) 男人和欧体男人的分析.
西部斑点和流动细胞计量以评估TLR8蛋白水平.

主要成果:

与TLR7相似的TLR8在人类免疫细胞中避开XCI.
具有TLR7和TLR8两种转录的细胞在雌性和KS雄性中相比于欧体雄性更为频繁.
在女性单核血细胞中,TLR8蛋白表达明显高于男性细胞.

结论:

在人类单细胞和CD4+T细胞中,TLR8逃脱了XCI,反映了TLR7.
相互依赖的转录和XCI逃逸可能会导致女性的TLR8蛋白增加.
这些发现对病毒/细菌反应和自身免疫性疾病易感性有影响.

关键词:

人体单细胞和CD4+T细胞克莱因费尔特综合征是什么意思收费类受体 7 收费类受体 7 收费类受体 8 收费类受体 8 脱离X染色体失活的方法

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