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对巨细胞碳amylated蛋白质组的分析揭示了一个功能在翻译后修饰交叉通话的功能

Youngki You¹, Chia-Feng Tsai¹, Rishi Patel²

¹Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99352, USA.

Cell communication and signaling : CCS

|September 18, 2023

概括

用一种新的方法系统分析了风湿性关节炎和脏疾病的生物标志物氨酸碳amylation. 这项研究揭示了碳amylation.

科学领域:

生物化学生化学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

氨酸碳化是类风湿性关节炎和脏疾病的重要生物标志物.
由于缺乏有效的分析工具,对这种翻译后修饰 (PTM) 的细胞功能仍未得到充分研究.

研究的目的:

开发和验证一种用于系统分析氨酸碳amylation的新方法.
为了研究细胞功能和carbamylation与其他翻译后修饰 (PTMs) 的潜在交叉声.

主要方法:

通过使用抗乙氨酸抗体进行适应的协同亲和性净化,用于碳amylated 丰富.
采用固定金属 afinity 染色学用于聚丰富,以实现多PTM 分析.
在用脂聚糖处理的RAW 264.7巨细胞上测试了管道.

主要成果:

确定了7,299个乙化,8,923个碳化和47,637个化.
与乙化相比,碳化发生在各种功能性蛋白质上,具有不同的动机.
鉴定了1,183种通过乙化,碳化和酸化的蛋白质,54种由脂多糖调节,并富含免疫信号,包括无素-蛋白酶体通路.
证明了线性二维基因的碳amylation 抑制了二维基因酶OTULIN.

结论:

抗乙氨酸抗体对碳甲基化丰富是有效的.
碳amylation 参与 PTM 交叉与乙化和酸化.
碳amylation 在试验室中起到调节ubiquitination的作用.

关键词:

细胞信号传递细胞信号传递氨酸的碳amylation 氨酸的碳amylation 氨酸的多个omics的多个omics.后翻译修改后的修改.翻译后修改交叉对话

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