番茄果实衍生的细胞外囊泡通过脂质介导机制抑制菌核素
Bao-Hong Lee1, She-Ching Wu2, Hao-Yuan Chien3
1Department of Horticulture, National Chiayi University, Chiayi 60004, Taiwan.
Food & function
|September 19, 2023
概括
番茄果实细胞外囊泡 (EV) 促进有益的肠道细菌并抑制病原体. 这些来自植物的EV显示出治疗肠道失生症和预防感染的潜力.
科学领域:
- 植物生物学 植物生物学
- 微生物学 微生物学
- 纳米技术 纳米技术
背景情况:
- 细胞外囊泡 (EVs) 对于细胞间通信至关重要.
- 来自食用植物的电动汽车因其生物作用而受到越来越多的关注.
- 番茄果EVs被调查其对肠道微生物群的影响.
研究的目的:
- 为了隔离和描述番茄果实EVs.
- 分析番茄EVs对有益和致病性肠道细菌的影响.
- 为了研究番茄EVs对肠道疾病的治疗潜力.
主要方法:
- 通过超离心法从番茄果实中分离EVs.
- 对番茄电动车的物理,形态和生物载荷概况的分析.
- 在体外测试以评估对*Lactobacillus*,*Clostridioides difficile*和*Fusobacterium nucleatum*的影响.
- 肠道微生物群发酵模型,以评估反转失生症.
- 脂质分析和操纵实验以确定关键的抗菌成分.
主要成果:
- 番茄电动汽车成功地被隔离和特征化.
- 番茄EVs促进了Lactobacillus物种的生长.
- 番茄EVs抑制了*C. difficile*和*F. nucleatum*的生长.
- 番茄EVs在肠道模拟器中逆转了*F.核*诱导的微生物群失调.
- 番茄EV中特定的脂质被确定为抗菌作用的原因.
结论:
- 番茄衍生的EVs对肠道微生物群调节具有有益的特性.
- 来自植物的电动汽车显示出作为治疗肠道失调症的新治疗策略的希望.
- 抗菌作用归因于EVs中的特定脂质成分.
- 番茄电动汽车有可能预防肠道细菌感染并恢复肠道健康.
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