在不同发育阶段的雅克丸中,ET-1和eNOS表达的比较
Juanjuan Song1,2,3, Ligang Yuan1,2, Wang Hua1,2
1College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
Reproduction in domestic animals = Zuchthygiene
|September 19, 2023
概括
雅克丸表达内甲蛋白-1 (ET-1) 和内甲氧化合成酶 (eNOS) 的年龄不同,老年雅克的水平更高. 这些因素对雅克丸发育和低氧环境中的微循环至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 生理学 生理学 生理学
- 环境适应 环境适应
背景情况:
- 雅克对高海拔,低氧环境有着显著的适应能力.
- 内甲素-1 (ET-1) 和内甲氧化合成酶 (eNOS) 是血液氧气运输的关键调节剂.
- 了解雅克的丸功能对于他们在具有挑战性的气候下繁殖成功至关重要.
研究的目的:
- 在雅克丸中研究ET-1和eNOS与年龄相关的表达模式.
- 阐明ET-1和eNOS在高原条件下的雅克丸发育和微循环中的作用.
主要方法:
- 微观分析包括H&E染色,免疫组织化学和免疫光.
- 西方斑块用于蛋白质表达分析.
- 实时定量聚合酶连锁反应 (RT-qPCR) 用于mRNA表达分析.
主要成果:
- 雅克丸中的ET-1和eNOSmRNA和蛋白质水平随着年龄的增长而增加,在老雅克中最高.
- ET-1和eNOS被定位在新生儿的血球细胞和精子细胞膜中,年轻和成年雅克的莱迪格细胞和内皮细胞,老雅克的塞尔托利细胞/毛细血管.
- 表达模式表明参与丸发育,莱迪格和塞尔托利细胞功能和微循环调节.
结论:
- 根据年龄,ET-1和eNOS的表达对雅克丸的发育和功能至关重要.
- 这些因素在调节雅克丸内的微循环中起着重要作用,特别是在对低毒环境的反应中.
- 这些发现提供了对高海拔地区,寒冷和缺氧条件下雅克繁殖生理学的见解.
相关概念视频
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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...


