血清中维生素A和E的质量保证计划:我们是否做得足以评估实验室的性能?
Michael Fitzpatrick1,2, Trisha Andersen1,3, Jonathan Bush1,4
1AACB/RCPAQAP Vitamins Advisory Committee, St Leonards, NSW, Australia.
Clinical chemistry and laboratory medicine
|September 19, 2023
概括
维生素E类似物会干扰血清中的视网醇和α-托哥菲醇测量,可能误导营养状况. 实验室应审查分析方法,以确保准确的维生素量化.
科学领域:
- 临床化学 临床化学
- 营养科学 营养科学
- 实验室医学 实验室医学
背景情况:
- 血清维生素A (视网醇) 和维生素E (α-托哥菲醇) 的监测评估了营养状况.
- 精确的测量是至关重要的,但由于干扰物质而具有挑战性.
- 现有的质量保证计划可能不包括在患者样本中发现的维生素类似物.
研究的目的:
- 为了评估实验室在定量雷丁醇和α-托科菲罗尔的准确性.
- 评估维生素E类似物 (多科菲罗酸盐,γ-多科菲罗) 对这些测量结果的影响.
- 识别营养状况评估中的潜在不准确性.
主要方法:
- 在参与实验室中分发了一系列补充的对照和增值样本.
- 雷丁醇和α-托科菲罗尔的结果与对照样本进行了比较.
- 性能根据RCPAQAP允许的性能规范 (APS) 进行评估.
主要成果:
- 来自α-托哥法醇的干扰影响了30%的雷丁醇实验室和23%的α-托哥法醇实验室.
- 一个实验室报告说,在存在γ-托科菲罗尔时,α-托科菲罗尔的结果不准确.
- 结果表明营养状况可能被高估或低估.
结论:
- 维生素E类似物可以显著影响血清维生素A和E测量的准确性.
- 实验室需要审查分析程序以减轻干扰.
- 外部质量保证提供者应考虑使用增值样本来更好地评估实验室能力.
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