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对人类肠热的早期转录反应具有挑战性

Amber Barton¹, Jennifer Hill¹, Daniel O'Connor¹

¹Oxford Vaccine Group, Department of Paediatrics, University of Oxford and the NIHR Oxford Biomedical Research Centre , Oxford, United Kingdom.

Infection and immunity

|September 19, 2023

概括

对肠道发烧的早期免疫反应沙门氏菌感染可在12小时内检测到,涉及中性粒细胞和单细胞的基因表达变化. 接种疫苗并没有改变这种初始的先天性免疫反应.

科学领域:

传染性疾病传染性疾病
免疫学免疫学免疫学
基因组学就是基因组学.

背景情况:

由伤寒沙门氏菌引起的肠热是一种发烧性疾病,具有很长的潜伏期.
了解这个潜伏期早期的先天免疫反应对于开发有效的干预措施至关重要.

研究的目的:

通过使用人类模型,研究肠热挑战后的早期先天性免疫反应.
评估用Vi多糖或合疫苗接种疫苗如何影响这种早期反应.

主要方法:

使用了人类挑战模型与伤寒型沙门氏菌.
在挑战后12小时分析了外周血液转录组.
测量的血瘤坏死因子-α (TNF-α).
根据基因表达数据估计的免疫细胞比例.

主要成果:

在挑战后12小时内,外围血液转录组中的199个基因被上调.
与中性粒细胞,单细胞和先天免疫相关的基因组被过度代表.
观察到活化单细胞的潜在增加和血TNF-α的升高.
在发烧和接种疫苗组之间,没有发现基因表达的显著差异.

结论:

周围血液转录组的早期扰乱发生在肠热发作12小时内.
这项研究提供了初步的洞察力,了解了早期的先天性免疫机制与肠炎有关.
在这个模型中,早期反应没有受到疫苗接种状态的显著改变.

关键词:

沙门氏菌是一种沙门氏菌.肠道发烧肠道发烧这是人类面临的挑战.文字转录字体转录字体转录伤寒是什么意思? 伤寒是什么意思?

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