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开发一种SNP标记器集,用于使用四状里亚基因型测序来进行标记器辅助的逆交
Jae-Eun Oh1,2, Ji-Eun Kim1, Jangmi Kim1
1SEEDERS Inc, Daejeon, 34912, Republic of Korea.
Molecular genetics and genomics : MGG
|September 19, 2023
概括
研究人员开发了一套全基因组的竞争性等位基因特异性PCR (KASP) 标记物,用于鱼繁殖. 这在这个重要的作物中加速了标记器辅助循环选择 (MARS).
科学领域:
- 植物遗传学 植物遗传学
- 提高作物质量 提高作物质量
- 分子育种是一种分子育种.
背景情况:
- 高质量的分子标记物对于通过标记物辅助选择加速作物育种至关重要.
- 多倍体作物,如,由于具有相似的同源子基因组,在开发特定位置的单核酸多态性 (SNP) 分析方面存在挑战.
- 对于缺乏参考基因组的物种来说,创建全基因组标记集是很困难的.
研究的目的:
- 开发一套全基因组的竞争性等位基因特异性PCR (KASP) 标记物,用于四状中标记辅助复发性选择 (MARS).
- 使用基因型测序 (GBS) 建立高分辨率的鱼遗传地图.
- 为了验证开发的KASP标记的准确性.
主要方法:
- 用基因型测序 (GBS) 来构建来自两个F2鱼种群的高分辨率链接地图.
- 整合了两个遗传地图,以创建一个最终的地图,其中包括22个链接组和1123个共同标记.
- 为MARS选择了一组96个全基因组标记,并使用Fluidigm系统确认了它们的准确性.
主要成果:
- 创建了两个高分辨率的链接地图,包括2326个和2454个SNP标记.
- 建立了一个最终的遗传地图,包含22个链接组.
- 与GBS数据相比,在选择的KASP标志物中确认了91.8%的准确性.
结论:
- 这项研究成功地开发了一套针对perilla的全基因组KASP标记物.
- 这些标记物为标记物辅助的逆交和开发新的Perilla品种提供了基础.
- 这些发现有助于在这种次要作物中加速繁殖进展.
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