植物激素调节无性异性Toxoplasma gondii的复制
Tina Wagner1, Berit Bangoura2, Stefanie Wiedmer3
1Institute of Inflammation and Neurodegeneration, Otto-von-Guericke University Magdeburg, Magdeburg, Germany.
Parasitology research
|September 19, 2023
概括
酸 (ABA) 显著增加了东道细胞中Toxoplasma gondii的复制. 吉伯酸 (GIBB) 可能抑制寄生虫的生长,这表明对毒素菌的潜在治疗方法.
科学领域:
- 寄生虫学的寄生虫学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 毒淋巴菌 (T. gondii) 在人类和动物中引起系统性感染.
- 在急性感染期间,太基子细胞在细胞内复制,涉及入侵,繁殖和退出.
- 已知植物激素会影响介导分泌,影响寄生虫入侵.
研究的目的:
- 为了研究植物激素对T. gondii tachyzoite在人类前皮纤维细胞 (HFF) 培养中的复制作用.
- 为了评估abscisic acid (ABA), gibberellic acid (GIBB) 和kinetin (KIN) 对寄生虫复制的影响.
- 评估细胞培养基成分在调解植物激素效应中的作用.
主要方法:
- 通过使用定量聚合酶链反应 (qPCR) 测量寄生虫DNA拷贝来量化T. gondii的复制.
- 三种植物激素 (ABA,GIBB,KIN) 在T. gondii感染的HFF细胞培养物上进行了测试.
- 实验是在典型的补充细胞培养基中进行的,以模仿生理条件.
主要成果:
- 酸 (ABA) 在20 ng/μl和2 ng/μl的度下显著增加了T. gondii的DNA拷贝.
- 吉伯酸 (GIBB) 显示出作为T. gondii生长抑制剂的潜力,其作用取决于培养基成分.
- 基尼丁 (KIN) 作用没有明确详细说明,但是测试植物激素的一部分.
结论:
- 酸 (ABA) 在特定条件下促进T. gondii在宿主细胞中的复制.
- 吉伯酸 (GIBB) 值得进一步研究,因为它是抗毒素菌的潜在治疗剂.
- 植物激素与宿主寄生虫系统的相互作用为了解和治疗寄生虫感染提供了新的途径.
更多相关视频
11:21Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
Published on: March 12, 2015
11.1K
11:37QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
Published on: June 22, 2017
16.3K
相关概念视频
Gene Regulation During Sporulation
34
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
34
Hormonal Regulation
43.6K
Hormones regulate a significant portion of digestion through activation of the neuroendocrine system. The neuroendocrine system of digestion contains many different hormones all with multiple functions that are both, directly and indirectly, involved in digestion.
43.6K
Molecular Factors Affecting Cell Division
3.2K
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
3.2K
Negative Regulator Molecules
35.4K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.4K
Background and Environment Affect Phenotype
6.6K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.6K
Epigenetic Regulation
31.1K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
31.1K
