从波兰蜜蜂花粉和蜜蜂面包中分离的Bacillus的益生菌潜力
Karolina Pełka1, Ahmer Bin Hafeez1, Randy W Worobo2
1Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, Narutowicza 11/12, 80233, Gdansk, Poland.
Probiotics and antimicrobial proteins
|September 19, 2023
概括
蜜蜂面包和蜜蜂花粉含有具有益生菌潜力的细菌菌株. 这些安全有效的菌株表现出抗菌性质和胆固醇同化,突出显示了它们在功能性食品中的价值.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 益生菌 益生菌 益生菌
背景情况:
- 蜜蜂花粉和蜜蜂面包是富含微生物的天然产品.
- 细菌物种以其潜在的益生菌特性和工业应用而闻名.
- 评估益生菌菌株的新来源对于开发功能性食品和治疗剂至关重要.
研究的目的:
- 评估从蜜蜂面包和蜜蜂花粉中分离出来的Bacillus菌株的益生菌潜力.
- 评估潜在益生菌应用所选菌株的安全性和功能特性.
- 通过基因组分析识别和描述有前途的益生菌候选人.
主要方法:
- 基于对 Staphylococcus aureus 和 Escherichia coli 的对抗性,对 Bacillus 菌株进行隔离和选择.
- 评估益生菌的标准:安全性 (溶血活性),在压力条件下的活力 (胆盐,酸),粘附性 (疏水性,自动和协同聚合),和胆固醇的同化.
- 抗生素敏感性测试. 抗生素敏感性测试.
- 全基因组测序用于物种识别和分析抗微生物化合物生产潜力.
主要成果:
- 三种细菌菌株 (PY2.3,BP20.15,BB10.1) 显示出显著的益生菌潜力.
- 所有选择的菌株都是非溶血的,经受了胆盐和酸的条件,表现出粘附性质和同化胆固醇.
- 基因组测序确定了细菌的细菌菌株 (BP20.15,BB10.1) 和细菌 velezensis (PY2.3).
- 基因组分析表明,有可能产生细菌素和其他抗微生物化合物.
结论:
- 蜜蜂花粉和蜜蜂面包中的细菌菌株具有宝贵的益生菌特性.
- 这些菌株符合益生菌的关键安全性和功能要求.
- 基因组洞察证实了它们在生产抗微生物物质方面的潜力,并建议在食品和卫生领域的应用.
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