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在人类结肠发酵模型中评估四种多种类的益生菌尾酒

Elizabeth Goya-Jorge¹, Irma Gonza¹, Pauline Bondue¹

¹Laboratory of Food Quality Management, Department of Food Sciences, FARAH - Veterinary Public Health, University of Liège, B43b, 4000, Liège, Belgium.

Probiotics and antimicrobial proteins

|September 19, 2023

概括

这项研究探讨了多菌株益生菌尾酒的肠道健康. 一种含有Bacillus subtilis,Levilactobacillus brevis和Lacticaseibacillus paracasei的混合物显著增加了丁酸盐的生产,并显示了进一步研究的潜力.

科学领域:

微生物学微生物学
胃肠病学胃肠病学
免疫学免疫学免疫学

背景情况:

细菌疗法具有治疗潜力,但多菌株益生菌组合仍未得到充分探索.
益生菌尾酒可能为疾病预防和治疗提供多目标方法.

研究的目的:

为了评估四种不同的三菌株益生菌混合物对人肠道微生物群的 in vitro 影响.
确定有希望的益生菌配方来调节肠道微生物群和相关宿主反应.

主要方法:

使用Bacillus coagulans,Levilactobacillus brevis,Lacticaseibacillus paracasei,Bacillus subtilis亚种制备了四种益生菌尾酒. 纳托,菌,和生物细菌动物亚种. 牛奶. 牛奶. 牛奶. 这是.
在体外模拟人类结肠微生物群和肠道上皮细胞模型.
分析丁酸盐的产生,细菌殖民,AhR的交换性,体电阻 (TEER) 和细胞因子的表达.

主要成果:

与对照组相比,所有测试的益生菌混合物都显著增加了丁酸盐的产量.
益生菌尾酒PA和PB显示出双生菌效应,并促进了乳球菌的殖民.
来自PA和PB的发酵超剂诱导了AhR的交换性;PB的代谢输出在Caco-2细胞中调节了关键细胞因子和AhR相关基因.

结论:

多菌株益生菌混合物可以有效调节肠道微生物群,显著增加丁酸盐的产生.
一个特定的配方 (Bacillus subtilis亚种. natto,Levilactobacillus brevis,Lacticaseibacillus paracasei) 显示了进一步研究的巨大潜力.
益生菌尾酒是开发有针对性的肠道健康干预措施的一个有希望的途径.

关键词:

结肠中的微生物群胃肠道模拟胃肠道模拟我们的肠道微生物群.肠道细胞培养的方法多种菌株的多种菌株.益生菌益生菌益生菌

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